Overview

Also Known As:RARbeta-

This Rarb knockout strain is useful in studies of synaptic plasticity and the role of retinoic acid receptors during development.

Donating Investigator

Henry Sucov, Medical University of South Carolina

Genetic overview

Genetic Background	Generation

Rarb^tm1Vgi

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Rarb	retinoic acid receptor, beta

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Research Applications

Research Tools
Neurobiology Research
Developmental Biology Research
Internal/Organ Research
Endocrine Deficiency Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Rarb (retinoic acid receptor, beta) gene encodes for a nuclear ligand-dependent transcription factor and is expressed in the developing and adult mouse, particularly in the developing lung and central nervous system. These mice carry a targeted mutation for the Rarb gene in which a NEO cassette replaces exon 6 (encoding the first zinc finger). Mice that are heterozygous for the targeted mutation and on a mixed STOCK background are viable and fertile; it is not known if homozygotes on the STOCK background are viable. On the B6;129 background homozygotes are viable and fertile. No gene product (mRNA) is detected by Northern blot analysis. Homozygotes on the B6;129 background exhibit impaired spatial learning and memory with an absence of hippocampal CA1 region long-term potentiation and long-term depression. When compared to wildtype mice, homozygotes have reduced respiratory function and delayed mammary gland duct development.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 6, which encodes the first zinc finger. The construct was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to several other mutant lines with genetic backgrounds including, but not limited to: C57BL/6, ICR (CD-1), and 129Sv. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish this colony of mice carrying only the Rarb^tm1Vgi allele.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Luo J; Pasceri P; Conlon RA; Rossant J; Giguere V. 1995. Mice lacking all isoforms of retinoic acid receptor beta develop normally and are susceptible to the teratogenic effects of retinoic acid. Mech Dev 53(1):61-71PubMed: 8555112 MGI: J:29061

View All References

Genetics

Rarb^tm1Vgi

Allele Symbol: Rarb^tm1Vgi

Allele Name	targeted mutation 1, Vincent Giguere
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	RARbeta-
Gene Symbol and Name	Rarb, retinoic acid receptor, beta
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	14
Molecular Note	A genomic fragment containing sequences encoding the first zinc finger motif was replaced with a neomycin selection cassette. This region encodes an essential portion of the protein common to all encoded isoforms. Northern blot analysis on RNA derived from E13.5 homozygous embryos demonstrated that no detectable transcript is produced from this allele.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
Neurobiology Research
- Behavioral and Learning Defects
Developmental Biology Research
- Internal/Organ Defects
  - lung
Internal/Organ Research
- Lung Defects
Endocrine Deficiency Research
- Mammary Gland Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Rarb^tm1Vgi/Rarb^tm1Vgi
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

abnormal spatial learning
- d type controls
- mutants display severe impairment in the Morris water maze test and show no improvement in performance over a training period of 8 days; after 24 hours mutants spend less time in the water where the platform had been in the previous trials compared to wild type controls

cardiovascular system phenotype

decreased pulmonary endothelial cell surface
- the gas-exchange surface area per cm3 of mutant lung tissue is reduced by ~30% at P28, P42 and P56

growth/size/body region phenotype

decreased body weight
- Normal - however, no significant differences in body weight are observed at P42 and P56
- slightly but significantly decreased body weights are noted at P28

nervous system phenotype

abnormal excitatory postsynaptic potential
- only transient depression of field excitatory postsynaptic potentials is observed in mutants in response to low frequency stimulation, while the controls show a long-lasting depression

absent long term depression
- long term depotentiation can not be induced in mutants

reduced long term potentiation
- long term potentiation is absent in hippocampal slices from mutants

respiratory system phenotype

abnormal lung development
- impaired formation of the distal airspaces during the postnatal phase of lung maturation

abnormal pulmonary alveolar duct morphology
- alveolar ducts comprise a significantly greater proportion of mutant lung tissue at P28, P42, and P56

abnormal pulmonary alveolar sac morphology
- alveolar sacs comprise a significantly greater proportion of mutant lung tissue at P28, P42, and P56

abnormal pulmonary alveolar system morphology
- Normal - however, the mean volume of individual alveoli, number of alveoli per cm3 of lung tissue, and distribution of alveolar volumes remain relatively normal at all postnatal time points studied
- Normal - no differences in the percentage of BrdU-labeled cells are noted in the alveolar region at any of time point
- Normal - no significant difference in the thickness of alveolar walls is observed at any time point
- the mean cord length (Lm) of mutant respiratory airspaces is significantly increased only at P42
- the volume density of airspace in the alveolar region of mutant lungs is significantly increased at P28, P42, and P56

abnormal respiratory function
- carbon monoxide (CO) uptake is significantly reduced in mutant lungs at P56 when mice are at rest
- Normal - however, the pressure/volume characteristics of the lung are not significantly altered at this time point

decreased pulmonary endothelial cell surface
- the gas-exchange surface area per cm3 of mutant lung tissue is reduced by ~30% at P28, P42 and P56

impaired lung alveolus development
- Normal - however, no differences in Pdgfra mRNA levels are observed at any postnatal age
- significantly reduced PDGFA protein levels are observed at P28, suggesting impaired postnatal lung alveolarization

References

Luo J; Pasceri P; Conlon RA; Rossant J; Giguere V. 1995. Mice lacking all isoforms of retinoic acid receptor beta develop normally and are susceptible to the teratogenic effects of retinoic acid. Mech Dev 53(1):61-71PubMed: 8555112 MGI: J:29061

Additional - Rarb^tm1Vgi related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Rarb
- Separated PCR:R26R Alternate1
- Standard PCR:Rara
- Standard PCR:Tgfbr2 fl
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). It is not known if homozygotes on a mixed STOCK background are viable.
- Additional Breeding and Husbandry Support
Citation
When using the RARbeta- mouse strain in a publication, please cite the originating article(s) and include JAX stock #022999 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Rarb<tm1Vgi>

Related Products and Services

Frozen Mouse Embryo	STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo	$3373.50
Frozen Mouse Embryo	STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Also Known As:RARbeta-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Rarbtm1Vgi

Allele Symbol: Rarbtm1Vgi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Rarbtm1Vgi/Rarbtm1Vgiinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological phenotype

cardiovascular system phenotype

growth/size/body region phenotype

nervous system phenotype

respiratory system phenotype

References

Additional - Rarbtm1Vgi related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Rarb^tm1Vgi

Allele Symbol: Rarb^tm1Vgi

Genotype: Rarb^tm1Vgi/Rarb^tm1Vgi
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - Rarb^tm1Vgi related