This Rarb knockout strain is useful in studies of synaptic plasticity and the role of retinoic acid receptors during development.
Henry Sucov, Medical University of South Carolina
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Rarb | retinoic acid receptor, beta |
Rarb (retinoic acid receptor, beta) gene encodes for a nuclear ligand-dependent transcription factor and is expressed in the developing and adult mouse, particularly in the developing lung and central nervous system. These mice carry a targeted mutation for the Rarb gene in which a NEO cassette replaces exon 6 (encoding the first zinc finger). Mice that are heterozygous for the targeted mutation and on a mixed STOCK background are viable and fertile; it is not known if homozygotes on the STOCK background are viable. On the B6;129 background homozygotes are viable and fertile. No gene product (mRNA) is detected by Northern blot analysis. Homozygotes on the B6;129 background exhibit impaired spatial learning and memory with an absence of hippocampal CA1 region long-term potentiation and long-term depression. When compared to wildtype mice, homozygotes have reduced respiratory function and delayed mammary gland duct development.
A targeting vector containing a NEO cassette was used to disrupt exon 6, which encodes the first zinc finger. The construct was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to several other mutant lines with genetic backgrounds including, but not limited to: C57BL/6, ICR (CD-1), and 129Sv. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish this colony of mice carrying only the Rarbtm1Vgi allele.
Allele Name | targeted mutation 1, Vincent Giguere |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | RARbeta- |
Gene Symbol and Name | Rarb, retinoic acid receptor, beta |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 14 |
Molecular Note | A genomic fragment containing sequences encoding the first zinc finger motif was replaced with a neomycin selection cassette. This region encodes an essential portion of the protein common to all encoded isoforms. Northern blot analysis on RNA derived from E13.5 homozygous embryos demonstrated that no detectable transcript is produced from this allele. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). It is not known if homozygotes on a mixed STOCK background are viable.
When using the RARbeta- mouse strain in a publication, please cite the originating article(s) and include JAX stock #022999 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Rarb<tm1Vgi> |
Frozen Mouse Embryo | STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Rarb<tm1Vgi>/HsvJ Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.