These transgenic mice conditionally express mouse Thbs4 from a tetracycline-responsive element (TRE; tetO) and mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha; also known as alpha MHC) minimal promoter. When combined with a tTA or rtTA allele, conditional, tissue-specific expression of THBS4 can be enabled.
Jeffery D. Molkentin, Cincinnati Children's HospitalRead More +
Endoplasmic reticulum (ER) stress response to tissue damage reduces protein synthesis in the ER, enhances protein degradation of damaged or misfolded proteins, and selectively induces expression of protective proteins. Thbs4 (thrombospondin 4) is one of the protective proteins upregulated in conjunction with ER stress responses in diseased hearts.
These transgenic mice conditionally express mouse Thbs4 from a tetracycline-responsive element (TRE; tetO) and mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha; also known as alpha MHC) minimal promoter. When combined with cardiac-specific Myh6-tTA, removal of dietary doxycyline enables overexpression of THBS4 in the heart and protects animals from protein aggregation-dependent cardiomyopathy. Double transgenic tetO/tTA mice show a large expansion of the ER and post-ER vesicles in the heart.
A transgenic vector expressing mouse Thbs4 from a tetracycline-responsive element (TRE; tetO) and mouse Myh6 minimal promoter was introduced to FVB embryos. This strain was maintained on an FVB genetic background by the donating lab.
|Expressed Gene||Thbs4, thrombospondin 4, mouse, laboratory|
|Site of Expression|
|Allele Name||transgene insertion 17.7, Jeffery Molkentin|
|Allele Type||Transgenic (Inducible, Inserted expressed sequence)|
|Gene Symbol and Name||Tg(tetO-Thbs4)17.7Jmol, transgene insertion 17.7, Jeffery Molkentin|
|Promoter||Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine|
|Promoter||tetO, tet operator,|
|Expressed Gene||Thbs4, thrombospondin 4, mouse, laboratory|
|Strain of Origin||Not Specified|
|Molecular Note||A transgene expresses mouse Thbs4 from a tetracycline-responsive element (TRE; tetO) and mouseMyh6 minimal promoter.|
|Please inquire about possible genotypes.|
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