Homozygous Dbi or ACBP (diazepam binding inhibitor) null embryos fail to develop past the 2.5 dpc morula (8-cell) stage. This strain may be useful for studying the role of the acyl-CoA intracellular lipid binding protein in embryonic development.
Ann Kier, Texas A&M University, College of Veterinary Medicine and Biomedcial Sciences
Dbi or ACBP (diazepam binding inhibitor) encodes an intracellular lipid binding protein that binds long-chain fatty acyl-CoAs (LCFA-CoAs). ACBP is involved in lipid and glucose metabolism, and is known to modulate several nuclear receptors. ACBP KO mice heterozygous for this null allele are viable and fertile. Homozygous embryos fail to develop past the 2.5 dpc morula (8-cell) stage. This strain may be useful for studying the role of ACBP in embryonic development.
A targeting vector containing neomycin resistance gene was used to replace the N-terminal promoter region, exon/intron 1, exon 2 and part of intron 2. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6NCr and offspring were backcrossed to C57BL/6NCr for 10 generations. Upon arrival, mice were bred to C57BL/6NJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Ann Kier|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Dbi, diazepam binding inhibitor|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A neomycin resistance cassette replaced exons 1 and 2. QRT-PCR confirmed the absence of transcript expression in E2.5 morulas.|
While maintaining a live colony, these mice are bred as heterozygotes; the mutation is embryonic lethal.
When using the B6N.129S6-Dbitm1Kier/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36975 in your Materials and Methods section.