This strain carries a targeted knockout of the Snai3 (snail homolog 3 (Drosophila); also known as Smuc) gene. Homozygous null mice do not exhibit any obvious developmental phenotype, suggesting this gene is not essential for embryogenesis in mice.
Thomas Gridley, Maine Medical Center Research Institute
The Snail gene family encodes zinc finger-containing transcriptional repressor proteins. Snai1 (snail homolog 1 (Drosophila)) and Snai2 (snail homolog 2 (Drosophila)) genes play critical roles during vertebrate embryogenesis as well as tumor progression and metastasis. Comparatively little is known about Snai3 (snail homolog 3 (Drosophila); also known as Smuc), which is predominantly expressed in mouse skeletal muscle and thymus as early as embryonic day 13.5 (E13.5). It is also expressed in heart, lung, and spleen.
This strain carries a targeted knockout of Snai3. Homozygous null mice are viable and fertile and do not exhibit any obvious developmental phenotype. Results suggest this gene is not essential for embryogenesis in mice.
An FRT-neo-FRT-loxP cassette was introduced to intron 1 of the targeted gene and a second loxP site was place 5' of exon 1. The mutation was created by homologous recombination in 129S1/Sv-p+Tyr+Kitl+-derived CJ7 embryonic stem (ES) cells. Resultant mice were crossed with Meox2-Cre deleter animals (see Stock No. 003755) to excise exon 1 and the neomycin cassette. This strain was backcrossed to C57BL/6J for seven generations by the donating laboratory.
|Allele Name||targeted mutation 2.1, Tom Gridley|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Snai3, snail family zinc finger 3|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||An FRT-neo-FRT-loxP cassette was introduced to intron 1, and a second loxP site was place 5' of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. QRT-PCR confirmed the absence of transcript expression in embryos.|
Homozygotes and heterozygotes are viable and fertile.
When using the B6.129S-Snai3tm2.1Grid/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022871 in your Materials and Methods section.