B6;129S4-Dab2^tm1Cpr/J

Stock number: 022837
Research areas: Cancer Research, Research Tools

Description

These mice possess loxP sites flanking exon 2 of the disabled 2, mitogen-responsive phosphoprotein (Dab2) gene and have applications in studies related to the endocytosis, TGF-beta receptor signaling pathway and T reg cell function.

Detailed description

The Dab2 (disabled 2, mitogen-responsive phosphoprotein) gene encodes for a protein that is a putative tumor suppressor and that has roles in clathrin-mediated endocytosis, TGF-beta receptor signaling, Treg cell function, cellular differentiation and embryonic development. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in CD4+ T cells, this mutant mouse strain may be useful in studies of Treg cell regulation and the FOXP3 signaling pathway.

When crossed to B6.129S4-Meox2^tm1(cre)Sor/J (see Stock No. 003375) Cre recombinase expression in epiblast-derived tissues results in offspring that exhibit reduced clathrin-coated pits in kidney proximal tubule cells and excrete specific plasma proteins in the urine.

Development

A loxP site flanked targeting vector designed by Dr. Jonathan A. Cooper (Fred Hutchinson Cancer Research Center) and containing a PGK-neo cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Dab2^tm1Cpr
Name: targeted mutation 1, Jonathan A Cooper
MGI accession ID: MGI:2387166
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dab2
Marker name: disabled 2, mitogen-responsive phosphoprotein
Chromosome: 15
Strain of origin: 129S4/SvJaeSor
Molecular note: Exon 2, which encodes the phosphotyrosine binding (PTB) domain common to all known isoforms, was left flanked by single loxP sites after a floxed neo cassette was excised by in vitro cre mediated recombination.