In this strain, a neo cassette replaces exons 3-7 and intron 7 of the keratin 12 (Krt12) gene with a neomycin resistance (neo) cassette, abolishing gene expression. Keratin 12 is a type I intermediate filament chain keratin, expressed in corneal epithelia. Keratin 12 also pairs with Keratin 3 and forms intermediate filaments in the cornea. Heterozygous mice appear phenotypically normal. Homozygotes are viable and fertile. The cornea of these Ktr12-/- mice are fragile, and contain fewer cellular layers and fewer intermediate filaments.
A targeting vector was designed to replace exons 3-7 and intron 7 of the keratin 12 (Krt12) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to Black Swiss mice. These mice were backcrossed 20 generations to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Winston W Y Kao|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Krt12, keratin 12|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exons 3-7 and intron 7 were replaced with a neomycin resistance gene. Immunohistochemistry, Western blot, Northern blot and in situ hybridization confirmed lack of gene expression.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129P2(Cg)-Krt12tm1Wwk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022813 in your Materials and Methods section.