B6.129P2(Cg)-Krt12^tm1Wwk/J

Stock number: 022813
Research areas: Cell Biology Research, Developmental Biology Research, Sensorineural Research

Description

These Ktr12^-/- mice may be useful for studying the maintenance of the corneal epithelium.

Detailed description

In this strain, a neo cassette replaces exons 3-7 and intron 7 of the keratin 12 (Krt12) gene with a neomycin resistance (neo) cassette, abolishing gene expression. Keratin 12 is a type I intermediate filament chain keratin, expressed in corneal epithelia. Keratin 12 also pairs with Keratin 3 and forms intermediate filaments in the cornea. Heterozygous mice appear phenotypically normal. Homozygotes are viable and fertile. The cornea of these Ktr12^-/- mice are fragile, and contain fewer cellular layers and fewer intermediate filaments.

Development

A targeting vector was designed to replace exons 3-7 and intron 7 of the keratin 12 (Krt12) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to Black Swiss mice. These mice were backcrossed 20 generations to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Krt12^tm1Wwk
Name: targeted mutation 1, Winston W Y Kao
MGI accession ID: MGI:3051854
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Krt12
Marker name: keratin 12
Marker synonyms: K12; Ka12; Krt1-12; MECD1
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: Exons 3-7 and intron 7 were replaced with a neomycin resistance gene. Immunohistochemistry, Western blot, Northern blot and in situ hybridization confirmed lack of gene expression.