These floxed mutant mice possess loxP sites flanking exons 8 to 13 of the Ctnnb1 gene. This strain may be useful for generating conditional mutations in applications related to the study of epithelial polarity, development and Wnt signaling.
Wilhelmine (Mimi) de Vries, The Jackson Laboratory
Dr. Barbara Knowles, Institute of Medical Biology
β-catenin, encoded by the Ctnnb1 gene, is part of the canonical Wnt signaling pathway and plays a role in epithelial polarity, cell–cell adhesion, and gene transcription. PDZ domain-containing proteins interact and bind at the C-terminus of β-catenin.
These mice carry loxP sites flanking exons 8 to 13 of the Ctnnb1 gene. Cre recombinase mediated excision of exons 8 to 13 results in the deletion of the C-terminus of the protein (amino acids 361 to 692), generating the β-catenin 4A12 allele, in the cre expressing tissues. Removal of the floxed sequence creates a null allele. Mice that are homozygous for the floxed allele are viable and fertile.
When bred to a strain with Cre recombinase expression in the female germ line (see Stock No. 003651 for example), this mutant mouse strain may be useful in studies of early embryonic development.
A targeting construct was used to flank exons 8 to 13 with loxP sites and insert a FRT site flanked NEO cassette into intron 13. The construct was electroporated into C57BL/6 derived B6 line #693 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6-Tyrc-2/J blastocysts. The resulting chimeric animals were bred to C57BL/6-Tyrc-2/J mice to confirm germline transmission. The heterozygous floxed β-cateninFC mice were then intercrossed to generate homozygotes. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Barbara B Knowles|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Ctnnb1, catenin (cadherin associated protein), beta 1|
|Strain of Origin||C57BL/6J|
|General Note||ES cell line #693 (C57BL/6J-693).|
|Molecular Note||A targeting construct was used to flank exons 8 to 13 with loxP sites and insert an FRT-flanked NEO cassette into intron 13. The loxP flanked region is designed to remove amino acids 361 to 692, which encompasses a part of armadillo repeat 6 to a part of the C terminus.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the βCatCf floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #022775 in your Materials and Methods section.