In this strain, a neo cassette replaces replace 1.9 kb, including exon 2, of the solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (Slc7a2) gene, abolishing gene expression. These mice may be useful for studying the control of CAT2 over inflammation and disease progression.
Lesley Ellies, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Slc7a2 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 |
In this strain, a neo cassette replaces replace 1.9 kb, including exon 2, of the solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (Slc7a2) gene, abolishing membrane bound CAT2 activity. Mice homozygous for this Cat2-/- allele are viable and fertile. CAT2 is a membrane associated protein involved in the cellular uptake of cationic amino acids such as arginine, lysine and ornithine. CAT2 plays a regulatory role in the activation of macrophages. Arginine is a substrate for nitric oxide synthase (NOS) during the production of nitric oxide (NO). The release of NO by inflammatory cells contributes to the progression of diseases such as cancer, arthritis, inflammatory bowel disease, Crohn's disease, and atherosclerosis. CAT2 plays a role in controlling inflammation and IL-17 activation in an injury model of colitis. These Cat2-/- mice exhibit a reduction in NO biosynthesis in macrophages. They have increased numbers of memory T cells and activated dendritic cells in their lungs. When infected with Helicobacter pylori these mice display suppression of gastric macrophage levels, macrophage expression of iNOS, dendritic cell activation, and expression of granulocyte-colony stimulating factor. They exhibit altered cytokine expression.
A targeting vector was designed to replace 1.9 kb, including exon 2, of the solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 (Slc7a2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females, and these mice were bred together to generate a colony of Cat2-/- mice. These mice were backcrossed for at least 10 generations to C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Carol L MacLeod |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cat2 - |
Gene Symbol and Name | Slc7a2, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 8 |
Molecular Note | A neomycin resistance cassette replaced 1.9 kb of sequence, including exon 2. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Cat2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #022767 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Slc7a2<tm1Clm> |
Frozen Mouse Embryo | B6.129S7-Slc7a2<tm1Clm>/LellJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Slc7a2<tm1Clm>/LellJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Slc7a2<tm1Clm>/LellJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Slc7a2<tm1Clm>/LellJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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