A bacterial artificial chromosome (BAC), RPCI23-385H16, was used to isolate 144kb sequence containing the entire mouse Prox1 (prospero-related homeobox 1) gene. A 144kb portion of this BAC, containing only Prox1, was modified to include a red fluorescent protein, tdTomato, from the pClasper vector, between amino acid 5 and the Prox1 nuclear localization sequence. This construct was microinjected into fertilized C57BL/6J oocytes. Mice from founder line 12, containing 10 copies of the transgene, were bred to C57BL/6J mice to establish a colony of ProxTom mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J mice (Stock No. 000664) to establish a colony.

A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Six of the 27 markers throughout the genome were segregating with an unknown strain.

• Noncarrier
• 000664 C57BL/6J

Additional Information

• Considerations for Choosing Controls

• Truman LA; Bentley KL; Smith EC; Massaro SA; Gonzalez DG; Haberman AM; Hill M; Jones D; Min W; Krause DS; Ruddle NH. 2012. ProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets. Am J Pathol 180(4):1715-25PubMed: 22310467MGI: J:182015