Mice harboring the pR26-228 AR8-TA-mCherry reporter express mCherry in cells and tissues that receive SMAD2/3 mediated signaling induced by Nodal/Activin. This strain allows live monitoring of SMAD2/3-mediated signaling events seen in embryonic development as well as during regeneration and homeostasis in adults.
Mark A. Magnuson, Vanderbilt University School of Medicine
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Mice harboring the pR26-228 AR8-TA-mCherry reporter are viable and fertile, with expression of mCherry under the control of the activin response element from the Xenopus laevis mix1b (Mix.2) promoter. The red fluorescent protein, mCherry, is expressed in cells and tissues that receive SMAD2/3 mediated signaling induced by Nodal/Activin. The Nodal signal transduction pathway is involved in mesoendoderm induction, patterning of the nervous system, and determination of dorsal- ventral axis in vertebrate embryos.
This strain allows live monitoring of
SMAD2/3-mediated signaling events seen in particular lineages and
stages in embryonic development as well as during regeneration and
homeostasis in adults. Additionally, it allows FACS-based isolation of
responding cells.
Using the ROSA26LCA or Loxed Cassette Acceptor allele (Gt(ROSA)26Sor tm1Mgn), which contains a loxP-flanked (lox71, lox2272) selection cassette in the ROSA26 promoter, a donor cassette is inserted into the acceptor allele via recombinase mediated cassette exchange (RMCE). The donor cassette or signaling sentinel construct includes a frt-flanked pgk-hygromycin and an activin response element (AR8) from the Xenopus laevis mix1b reporter fused to a TATA-mCherry reporter, which replaced the ROSA26 promoter sequence (-4 kb to -228 bp).
The construct was electroporated into 129S6/SvEvTac-derived TL1 ES cells. Correctly targeted ES cells were injected into blastocysts from C57BL/6J mice. Offspring were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice to delete the pgk-hygromycin cassette. The donating investigator reported that the mice were backcrossed to C57BL/6J mice for 7 generations prior to sending to MMRRC at JAX.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | Red fluorescent protein, mCherry, is expressed in cells and tissues involved in the Nodal signal transduction pathway (mesoendoderm induction, patterning of the nervous system, and determination of dorsal- ventral axis in vertebrate embryos). |
Allele Name | targeted mutation 2.1, Mark A Magnussen |
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Allele Type | Targeted |
Allele Synonym(s) | -228/AR8-TA-mCherry; Rosa26(228.AR8.TA.mCherry)Hri |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Promoter | mixl1, Mix paired homeobox, frog, western clawed |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Red fluorescent protein, mCherry, is expressed in cells and tissues involved in the Nodal signal transduction pathway (mesoendoderm induction, patterning of the nervous system, and determination of dorsal- ventral axis in vertebrate embryos). |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 6 |
General Note | This allele was generate from Gt(ROSA)26Sortm1Mgn. |
Molecular Note | This allele was created by RMCE in Gt(ROSA)26Sortm1Mgn. The original selections cassettes (puromycin N-acetyltransferase and a truncated form of HSV1 thymidine kinase and EM7-driven neomycin resistance) were replaced by a cassette composed of ROSA26 upstream sequence deleted from the start codon to -228, 8 copies of the 50-bp activin response element (ARE) from the Xenopus laevis Mix1b promoter controlling expression of the mCherry reporter. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase. |
While maintaining a live colony, these mice are bred as heterozygotes. The Donating Investigator has not attempted to make this strain homozygous for the targeted allele.
When using the B6.129S6(SJL)-Gt(ROSA)26Sortm2.1(mix1b-mCherry)Mgn/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36911 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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