The rd8 mutation, which is a characteristic of the C57BL/6N substrain, is corrected in these mice. Mice carrying the nuclease-mediated correction (emv1Mvw) do not exhibit either ELM fragmentation or outer retinal dysplasia. This strain may be useful for ocular phenotyping efforts requiring the C57BL/6N background
Wiles Michael, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Endonuclease-mediated | Crb1 | crumbs family member 1, photoreceptor morphogenesis associated |
The recessive mutation retinal degeneration 8 (rd8) is a strain characteristic of C57BL/6N-derived strains. Mice homozygous for the rd8 allele exhibit retinal external limiting membrane (ELM) fragmentation and outer retinal dysplasia. In this mouse, the rd8 mutation is corrected using TALEN-mediated homology-directed repair. Mice carrying the nuclease-mediated correction (emv1Mvw) do not exhibit either ELM fragmentation or outer retinal dysplasia. This strain may be useful for ocular phenotyping efforts requiring the C57BL/6N background.
The recessive mutation retinal degeneration 8 (rd8) is a strain characteristic of C57BL/6N-derived strains. The mutation is a single base deletion in exon 9 of the Crb1 gene that causes a frame shift resulting in a premature stop codon, which truncates the protein. Zygotes from C57BL/6NJ (Stock #005304) mice were targeted using a TALEN-mediated homology directed repair (HDR) pathway. TALEN targeting of exon 9 for HDR with single stranded oligonucleotides (ssODNs) replaces the deleted nucleotide (C/G) in the rd8 mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions. The founder mouse was crossed to C57BL/6NJ and offspring were maintained as a cross to C57BL/6NJ least 4 generations.
Allele Name | endonuclease-mediated revertant 1, Michael Wiles |
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Allele Type | Endonuclease-mediated |
Allele Synonym(s) | corrected; Crb1cor; Crb1em1Mvw |
Gene Symbol and Name | Crb1, crumbs family member 1, photoreceptor morphogenesis associated |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NJ |
Chromosome | 1 |
Molecular Note | TALEN targeting of exon 9 for homologous recombination with single stranded oligonucleotides (ssODNs) replaces the deleted nucleotide (C/G) in the retinal degeneration 8 (rd8) mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Crb1cor mouse strain in a publication, please cite the originating article(s) and include JAX stock #022521 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Crb1<rd8+em1Mvw>/ |
Frozen Mouse Embryo | C57BL/6NJ-Crb1<rd8+em1Mvw>/MvwJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6NJ-Crb1<rd8+em1Mvw>/MvwJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6NJ-Crb1<rd8+em1Mvw>/MvwJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6NJ-Crb1<rd8+em1Mvw>/MvwJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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