C57BL/6NJ-Crb1^rd8+em1Mvw/MvwJ

Stock number: 022521
Product name: Crb1^cor
Research areas: Sensorineural Research

Description

The rd8 mutation, which is a characteristic of the C57BL/6N substrain, is corrected in these mice. Mice carrying the nuclease-mediated correction (emv1Mvw) do not exhibit either ELM fragmentation or outer retinal dysplasia. This strain may be useful for ocular phenotyping efforts requiring the C57BL/6N background

Detailed description

The recessive mutation retinal degeneration 8 (rd8) is a strain characteristic of C57BL/6N-derived strains. Mice homozygous for the rd8 allele exhibit retinal external limiting membrane (ELM) fragmentation and outer retinal dysplasia. In this mouse, the rd8 mutation is corrected using TALEN-mediated homology-directed repair. Mice carrying the nuclease-mediated correction (emv1Mvw) do not exhibit either ELM fragmentation or outer retinal dysplasia. This strain may be useful for ocular phenotyping efforts requiring the C57BL/6N background.

Development

The recessive mutation retinal degeneration 8 (rd8) is a strain characteristic of C57BL/6N-derived strains. The mutation is a single base deletion in exon 9 of the Crb1 gene that causes a frame shift resulting in a premature stop codon, which truncates the protein. Zygotes from C57BL/6NJ (Stock #005304) mice were targeted using a TALEN-mediated homology directed repair (HDR) pathway. TALEN targeting of exon 9 for HDR with single stranded oligonucleotides (ssODNs) replaces the deleted nucleotide (C/G) in the rd8 mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions. The founder mouse was crossed to C57BL/6NJ and offspring were maintained as a cross to C57BL/6NJ least 4 generations.

Allele

Symbol: Crb1^rd8+em1Mvw
Name: endonuclease-mediated revertant 1, Michael Wiles
MGI accession ID: MGI:5498686
Generation method: Endonuclease-mediated
Synonyms: corrected; Crb1^cor; Crb1^em1Mvw
Marker symbol: Crb1
Marker name: crumbs family member 1, photoreceptor morphogenesis associated
Marker synonyms: 7530426H14Rik; A930008G09Rik; CRB1-A; CRB1-B; CRB1-C; LCA8; RP12
Chromosome: 1
Strain of origin: C57BL/6NJ
Molecular note: TALEN targeting of exon 9 for homologous recombination with single stranded oligonucleotides (ssODNs) replaces the deleted nucleotide (C/G) in the retinal degeneration 8 (rd8) mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions.