B6.Cg-Plvap^tm1.1Rvst/J

Stock number: 022519
Research areas: Cardiovascular Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 2-5 of the Plvap gene. This strain may be useful for generating conditional mutations in applications related to the formation of stomatal and fenestral endothelial diaphragms, and microvascular permeability.

Detailed description

The plasmalemma vesicle associated protein Plvap gene encodes an endothelial membrane glycoprotein essential for proper stomatal and fenestral endothelial diaphragm formation. These mice possess loxP sites on either side of exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have 2 through 5 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of microvascular permeability.

When bred to mice carrying Tg(Cdh5-cre)1Spe (Stock No. 017968), Cre recombinase expression in vascular endothelial cells results in lethality (genetic background dependent), diaphragm loss and leakage of plasma proteins.

Development

A targeting vector containing a loxP site and a FRT flanked Pgk-EM7-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 5. This construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived v6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to transgenic mice (Stock No. 005703) expressing Flpe recombinase under the control of the human ACTB promoter to remove the selection cassette. Mice that retained the loxP site flanked exon 2-5 were then bred to C57BL/6J mice to remove the Flpe transgene. The mice were then backcrossed to C57BL/6J for at least 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Plvap^tm1.1Rvst
Name: targeted mutation 1.1, Radu V Stan
MGI accession ID: MGI:5473517
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Plvap
Marker name: plasmalemma vesicle associated protein
Chromosome: 8
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Molecular note: A loxP site was inserted upstream of exon 2 and a an FRT flanked pgk-EM7-neo cassette and loxP site were inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the pgk-EM7-neo cassette leaving exons 2 - 5 floxed.