In progesterone receptor-expressing neurons of these PRPL mice, beta-galactosidase is expressed in cell soma, and placental alkaline phosphatase (PLAP) is expressed in cell projections. The reporter gene expression mimics the endogenous Pgr (progesterone receptor) gene expression pattern.
Nirao Shah, Stanford University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Pgr | progesterone receptor |
The progesterone receptor is a nuclear steroid receptor, ligand activated transcription factor which regulates gene expression following progesterone hormone binding. These mice contain IRES-PLAP-IRES-nuclear lacZ inserted into the 3' untranslated region (UTR) of the Pgr (progesterone receptor) locus. Reporter gene expression mimics the endogenous Pgr gene expression pattern. In progesterone receptor-expressing neurons, cell soma can be labeled by the nuclear lacZ and cell projections can be labeled by PLAP, placental alkaline phosphatase. Beta-galactosidase activity is detected in the forebrain (hypothalamic nuclei, posterodorsal
medial amygdala, medial division of
the posteromedial bed nucleus of the stria terminalis, various cortical areas, basal ganglia, and the dentate
gyrus). Placental alkaline phosphatase activity is detected in neuronal fibers of the forebrain. Expression and function of Pgr is unaffected. Mice that are homozygous for the targeted mutation are viable and fertile.
A targeting vector designed by Dr. Nirao M. Shah (University of California, San Francisco) containing IRES-PLAP-IRES-lacZ with nuclear localization signal sequence, and a self-excising neo resistance cassette (ACN) was inserted into the 3' untranslated region (UTR) of the gene. The construct was electroporated into 129/SvEv derived ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred to C57BL/6J females. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
---|---|
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression |
Allele Name | targeted mutation 2, Nirao M Shah |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | PRPL |
Gene Symbol and Name | Pgr, progesterone receptor |
Gene Synonym(s) | |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S/SvEv |
Chromosome | 9 |
Molecular Note | A targeting construct consisting of an IRES-PLAP-IRES-lacZ (IRES, internal ribosomal entry site; PLAP, placental alkaline phosphatase and lacZ has a nuclear localization signal) sequence and a self-excising neo resistance cassette (ACN) was used to target the Pgr locus in ES cells. ES cell were injected into blastocysts to produce chimeras. The neo gene in the ACN cassette is self-excised during passage through the male germline so F1 progeny from crosses of chimeric males to wild-type C57BL/6J females had ACN deletion. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S-Pgrtm2Shah/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022517 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Pgr<tm2Shah> |
Frozen Mouse Embryo | B6.129S-Pgr<tm2Shah>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Pgr<tm2Shah>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Pgr<tm2Shah>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Pgr<tm2Shah>/J Frozen Embryo | $3373.50 |
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