B6.129S-Pgr^tm2Shah/J

Stock number: 022517

Description

In progesterone receptor-expressing neurons of these PR^PL mice, beta-galactosidase is expressed in cell soma, and placental alkaline phosphatase (PLAP) is expressed in cell projections. The reporter gene expression mimics the endogenous Pgr (progesterone receptor) gene expression pattern.

Detailed description

The progesterone receptor is a nuclear steroid receptor, ligand activated transcription factor which regulates gene expression following progesterone hormone binding. These mice contain IRES-PLAP-IRES-nuclear lacZ inserted into the 3' untranslated region (UTR) of the Pgr (progesterone receptor) locus. Reporter gene expression mimics the endogenous Pgr gene expression pattern. In progesterone receptor-expressing neurons, cell soma can be labeled by the nuclear lacZ and cell projections can be labeled by PLAP, placental alkaline phosphatase. Beta-galactosidase activity is detected in the forebrain (hypothalamic nuclei, posterodorsal medial amygdala, medial division of the posteromedial bed nucleus of the stria terminalis, various cortical areas, basal ganglia, and the dentate gyrus). Placental alkaline phosphatase activity is detected in neuronal fibers of the forebrain. Expression and function of Pgr is unaffected. Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector designed by Dr. Nirao M. Shah (University of California, San Francisco) containing IRES-PLAP-IRES-lacZ with nuclear localization signal sequence, and a self-excising neo resistance cassette (ACN) was inserted into the 3' untranslated region (UTR) of the gene. The construct was electroporated into 129/SvEv derived ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred to C57BL/6J females. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Pgr^tm2Shah
Name: targeted mutation 2, Nirao M Shah
MGI accession ID: MGI:5487681
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Pgr
Marker name: progesterone receptor
Chromosome: 9
Strain of origin: 129S/SvEv
Expressed genes: lacZ,beta-galactosidase,E. coli; ALPP,alkaline phosphatase, placental,human
Molecular note: A targeting construct consisting of an IRES-PLAP-IRES-lacZ (IRES, internal ribosomal entry site; PLAP, placental alkaline phosphatase and lacZ has a nuclear localization signal) sequence and a self-excising neo resistance cassette (ACN) was used to target the Pgr locus in ES cells. ES cell were injected into blastocysts to produce chimeras. The neo gene in the ACN cassette is self-excised during passage through the male germline so F1 progeny from crosses of chimeric males to wild-type C57BL/6J females had ACN deletion.