Overview

Also Known As:Enhancer HS746KO

In this knockout of the non-coding enhancer element (Rr23or HS746), mRNA expression of Msx1 in mouse embryos is diminished and adult skulls exhibit significant differences in skull morphology. This strain may be useful for downstream analysis of craniofacial development.

Donating Investigator

Axel Visel, Lawrence Berkeley National Laboratory

Genetic overview

Genetic Background	Generation

Rr23^tm1Axvi

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout, No functional change)	Rr23	regulatory region 23

View Genetics

Research Applications

Developmental Biology Research

View All Research Applications

Details

Detailed Description

The regulatory region 23 (Rr23 or HS746) is a non-coding enhancer element that controls expression of Msx1 (homeobox, msh-like 1), a gene involved in craniofacial development. qPCR analysis indicates a 1.5 fold down regulation of Msx1 in homozygous HS1431 E11.5 and E13.5 embryos. Analysis of the adult skulls from homozygous mice by micro-computed tomography reveals significant differences in skull morphology compared to wild type. Alterations in HS746 include a shortening of the face, a widening of the posterior neurocranium, a narrowing of the palate and shortening of the cranial base. This strain may be useful for downstream analysis of craniofacial development.

Development

A targeting vector containing a loxP-flanked PGKneo cassette was used to delete 1682 bp of the HS746 enhancer sequence. Transient Cre expression in targeted cells excised the neo cassette. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for approximately three generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Genetics

Rr23^tm1Axvi

Allele Symbol: Rr23^tm1Axvi

Allele Name	targeted mutation 1, Axel Visel
Allele Type	Targeted (Null/Knockout, No functional change)
Allele Synonym(s)	HS746^_
Gene Symbol and Name	Rr23, regulatory region 23
Gene Synonym(s)
Strain of Origin	129S6/SvEvTac
Chromosome	5
Molecular Note	The enhancer sequence was replaced with a floxed neo selection cassette which was subsequently removed by cre excision.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Craniofacial and Palate Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Rr23^tm1Axvi/Rr23^tm1Axvi
involves: 129S6/SvEvTac * C57BL/6J

skeleton phenotype

short basicranium
- shortening of the cranial base

abnormal neurocranium morphology
- increase in the width of the posterior neurocranium

abnormal cranium morphology
- micro-computed tomography analysis reveals significant differences in skull morphology compared to wild type

craniofacial phenotype

abnormal neurocranium morphology
- increase in the width of the posterior neurocranium

abnormal cranium morphology
- micro-computed tomography analysis reveals significant differences in skull morphology compared to wild type

narrow palate

short basicranium
- shortening of the cranial base

short face
- decrease of facial length

digestive/alimentary phenotype

narrow palate

growth/size/body region phenotype

narrow palate

short face
- decrease of facial length

References

Additional - Rr23^tm1Axvi related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Rr24alternate1
- Separated PCR:Rr23Alternate 1
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the B6;129S6-Rr23^tm1Axvi/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36888 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Enhancer HS746KO

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Rr23tm1Axvi

Allele Symbol: Rr23tm1Axvi

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Rr23tm1Axvi/Rr23tm1Axviinvolves: 129S6/SvEvTac * C57BL/6J

skeleton phenotype

craniofacial phenotype

digestive/alimentary phenotype

growth/size/body region phenotype

References

Additional - Rr23tm1Axvi related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Rr23^tm1Axvi

Allele Symbol: Rr23^tm1Axvi

Genotype: Rr23^tm1Axvi/Rr23^tm1Axvi
involves: 129S6/SvEvTac * C57BL/6J

Additional - Rr23^tm1Axvi related