In this strain, a neo cassette replaces exon 2 and the coding region of exon 3 of the cardiotrophin 1 (Ctf1) gene, abolishing gene expression. These mice may be useful for studying the role of cardiotrophin 1 in neurogenesis and metabolism.
Michael Sendtner, University of Wuerzburg
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ctf1 | cardiotrophin 1 |
In this ct-1-/- strain, a neo cassette replaces exon 2 and the coding region of exon 3 of the cardiotrophin 1 (Ctf1) gene, abolishing gene expression. CT-1, a member of the gp130 family of cytokines, is highly expressed in embryonic skeletal muscle and promotes the survival of developing motor neurons. Through its activation of phosphatidylinositol 3-kinase, CT-1 acts to regulate fat and glucose metabolism. CT-1 has also been implicated in bone formation and myocardial remodeling. Homozygous mice are viable and fertile. Ct-1-/- mice exhibit increased cell death of developing motoneurons in spinal cord and brainstem in mice between E14 and the first postnatal week. They also have few cortical astrocytes and show reduction in muscle strength. These mice also display decreased energy expenditure, mature-onset obesity, insulin resistance, and hypercholesterolemia.
A targeting vector was designed to replace exon 2 and the complete coding region of exon 3 of the cardiotrophin 1 (Ctf1) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. The donating investigator reports that these ct-1-/- mice were bred for at least 10 generations to C57BL/6J background (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Michael Sendtner |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ct-1 - |
Gene Symbol and Name | Ctf1, cardiotrophin 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 7 |
Molecular Note | A neomycin selection cassette replaced a genomic fragment spanning exon 2 and the complete coding region of exon 3. In situ hybridization studies on homozygous embryos demonstrated that no detectable transcript is expressed from this allele. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S2-Ctf1tm1Msd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022355 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ctf1<tm1Msd> |
Frozen Mouse Embryo | B6.129S2-Ctf1<tm1Msd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Ctf1<tm1Msd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Ctf1<tm1Msd>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Ctf1<tm1Msd>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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