This recessive spontaneous single amino acid substitution in the myosin motor domain results in the head tossing and abnormal gait commonly found in Myo7a mutants.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous (Not Specified) | Myo7a | myosin VIIA |
This bankstock may still be segregating for the Gata1tm6Sho mutation
Mice homozygous for Myo7ash1-13J display extensive head tossing and some head shaking and head bobbing. Their gait can be lower to the ground than normal with the feet held wider apart and the tail often held aloft. They also have an increased tendency to move backward. Circling behavior has not been noted and homozygotes can reproduce and live a normal lifespan.
The Myo5ash1-13J mutation arose spontaneously in the C.129S1(B6)-Gata1tm6Sho/J strain at The Jackson Laboratory. This mutation was maintained by backcross-intercross of homozygous mutant females to BALB/cByJ males for two cycles and was subsequently sibling intercrossed for three generations then again backcross-intercross bred for one cycle before sperm was cryopreserved from homozygous males that had been backcrossed a cumulative total of three times away from the mutant parent strain. Thus, the Gata1tm6Sho mutation may still be present in this frozen sperm and recovered pups will be genotyped for both mutations.
Allele Name | shaker 1, 13 Jackson |
---|---|
Allele Type | Spontaneous (Not Specified) |
Allele Synonym(s) | |
Gene Symbol and Name | Myo7a, myosin VIIA |
Gene Synonym(s) | |
Strain of Origin | C.129S1(B6)-Gata1tm6Sho/J |
Chromosome | 7 |
Molecular Note | A spontaneous C to T transition at position 98,097,488 of chromosome 7 (GRCm38/mm10) changes amino acid 212 from arginine to histidine (p.R212H). |
Due to their circling and head tossing behavior, homozygous males should not be present with the pups and heterozygous females are better mothers than are homozygous females.
When using the C.Cg-Myo7ash1-13J/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #022311 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Myo7a<sh1-13J>. |
Frozen Mouse Embryo | C.Cg-Myo7a<sh1-13J>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C.Cg-Myo7a<sh1-13J>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | C.Cg-Myo7a<sh1-13J>/GrsrJ | $3373.50 |
Frozen Mouse Embryo | C.Cg-Myo7a<sh1-13J>/GrsrJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.