This spontaneous frameshift, predicted to modify only the last of the four zinc finger domains in ZFP191, is valuable in the study of the transcriptional regulation in oligodendrocytes that is essential for myelination of axons in the central nervous system.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Zfp24 | zinc finger protein 24 |
Mice homozygous for the Zfp191hmcns-2J mutation have very little myelin in the central nervous system when assessed at four weeks of age, but no myelin deficiency in the peripheral nervous system. Homozygotes can be identified as early as fourteen days of age by a whole body tremor that is most pronounced when the mouse is moving. Both males and females have tonic seizures. The severity of the tremors becomes more pronounced as they age and they do not survive beyond four to five weeks of age. Because of this, this strain must be maintained using heterozygous breeders. A hearing deficit, which varies in expressivity, has also been attributed to this mutation in this strain.
A spontaneous, dominant mutation causing head tossing arose in the strain CByJ.129P2(B6)-Cd4tm1Mak/J and this mutant subline was maintained by continuous backcross to BALB/cByJ. This removed the Cd4tm1Mak mutation by generation N2 and at generation N7 a new mutation arose spontaneously that was subsequently characterized as the Zfp191hmcns-2J mutation. Because this recessive Zfp191hmcns-2J mutation causes death before breeding age this mutant subline was maintained by sibling intercrossing heterozygotes, which were proven by progeny testing. The dominant head tossing mutation was easily bred out by phenotypic selection. The CByJ(Cg)-Zfp191hmcns-2J/GrsrJ strain reached generation +F3 in 2013.
Allele Name | hypomyelinated central nervous system 2 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Zfp24, zinc finger protein 24 |
Gene Synonym(s) | |
Strain of Origin | BALB/cByJ |
Chromosome | 18 |
Molecular Note | This spontaneous two base pair deletion in Chromosome 18 postitions 24,014,204 and 24,014,205 (GRCm38), which falls within exon 4, causes a frameshift that results in the substitution of 20 new amino acids in place of the last 18 amino acids of this 368 residue protein, thereby altering the last of four zinc finger domains. |
When using the CByJ(Cg)-Zfp24hmcns-2J/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #022138 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Zfp191<hmcns-2J> |
Frozen Mouse Embryo | CByJ(Cg)-Zfp24<hmcns-2J>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | CByJ(Cg)-Zfp24<hmcns-2J>/GrsrJ | $2595.00 |
Frozen Mouse Embryo | CByJ(Cg)-Zfp24<hmcns-2J>/GrsrJ | $3373.50 |
Frozen Mouse Embryo | CByJ(Cg)-Zfp24<hmcns-2J>/GrsrJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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