CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. These mice may be useful for generating conditional mutations in CD4-expressing tissues.
Christopher B. Wilson, University of Washington
Genetic Background | Generation |
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000664 C57BL/6J |
N13
|
Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$236.78 Domestic price for female 4-week |
315.29 Domestic price for breeder pair |
CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. Hemizygotes are viable and fertile. Specifically, Cre recombinase expression is observed in CD4-expressing T cells during sequential stages of T cell development in lymphoid tissues. Cre expression commences at the very late double-negative stage and generally results in >99% deletion of loxP flanked genes by the double-positive stage of T-cell development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying CD4 T cell maturation and proliferation.
The CD4-Cre transgene was designed with the CD4 enhancer, promoter and silencer sequence driving the expression of a Cre recombinase gene. This transgene was injected into oocytes and mice from founders line 1 were bred to C57BL/6 mice. This strain had been maintained on a C57BL/6 background when it was obtained by Dr. Binfeng Lu at University of Pittsburgh. These mice were then bred to mice carrying a floxed-Eomes (eomesodermin homolog) mutation (see Stock No. 017293) and a Tbx21 (T-box 21) knockout allele (see Stock No. 004432 and 004648). The donating investigator reported that these triple mutants were backcrossed with C57BL/6 inbred mice for ten generations. These mice arrived at the Jackson Laboratory Repository as Stock No. 017336 and were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation. Subsequently, the floxed-Eomes allele and Tbx21 knockout allele were bred out of the colony, resulting in a colony of CD4-cre transgenic mice. These mice were backcrossed to C57BL/6J mice to generate this congenic strain (Stock No. 022071). A 32 SNP (single nucleotide polymorphism) panel analysis determined that all of the 27 markers throughout the genome suggest a C57BL/6 genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | T cells |
Allele Name | transgene insertion 1, Christopher B Wilson |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | CD4CRE; CD4cre; CD4-Cre; CD4-Cre+; CD4-creTg; CD4p-Cre; Tg(CD4-cre)1Cw1 |
Gene Symbol and Name | Tg(Cd4-cre)1Cwi, transgene insertion 1, Christopher B Wilson |
Gene Synonym(s) | |
Promoter | Cd4, CD4 antigen, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | T cells |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | Cre recombinase is expressed under the control of mouse Cd4 regulatory elements. The Cd4 enhancer, promoter and silencer collectively drive expression at sequential stages specifically of CD4+ T cell development. |
Mutations Made By | Christopher Wilson, University of Washington |
Mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for several generations using a marker-assisted, congenic approach to establish this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred to wild-type (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the CD4Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #022071 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Cd4-cre)1Cwi |
Frozen Mouse Embryo | B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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