B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ

Stock number: 022071
Product name: CD4Cre
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This strain ships with remnant UID MiniMax microchips implanted subcutaneously in the back. For more information on these RFID chips, including our FAQ, please visit our Mouse Identification page.

CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. These mice may be useful for generating conditional mutations in CD4-expressing tissues.

Detailed description

CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. Hemizygotes are viable and fertile. Specifically, Cre recombinase expression is observed in CD4-expressing T cells during sequential stages of T cell development in lymphoid tissues. Cre expression commences at the very late double-negative stage and generally results in >99% deletion of loxP flanked genes by the double-positive stage of T-cell development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying CD4 T cell maturation and proliferation.

Development

The CD4-Cre transgene was designed with the CD4 enhancer, promoter and silencer sequence driving the expression of a Cre recombinase gene. This transgene was injected into oocytes and mice from founders line 1 were bred to C57BL/6 mice. Founder 1 integrated on chromosome 3 at position 60335693-60341285 (qD) and contains at least 15 complete sequential copies of the transgenic construct (Westendorf K, etal, J Immunol Methods 2016 Sep;436:54-7). This strain had been maintained on a C57BL/6 background when it was obtained by Dr. Binfeng Lu at University of Pittsburgh. These mice were then bred to mice carrying a floxed-Eomes (eomesodermin homolog) mutation (see Stock No. 017293) and a Tbx21 (T-box 21) knockout allele (see Stock No. 004432 and 004648). The donating investigator reported that these triple mutants were backcrossed with C57BL/6 inbred mice for ten generations. These mice arrived at the Jackson Laboratory Repository as Stock No. 017336 and were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation. Subsequently, the floxed-Eomes allele and Tbx21 knockout allele were bred out of the colony, resulting in a colony of CD4-cre transgenic mice. These mice were backcrossed to C57BL/6J mice to generate this congenic strain (Stock No. 022071).

In ~2013, a 32 SNP (single nucleotide polymorphism) panel analysis determined that all of the 27 markers throughout the genome suggest a C57BL/6 genetic background.

From 2015-2020, the 48 SNP panel analyses performed on cohorts from the live colony show that all of the markers throughout the genome were C57BL/6 allele-type, with all 5 C57BL/6 substrain SNPs showing C57BL/6J allele-type.

Allele

Symbol: Tg(Cd4-cre)1Cwi
Name: transgene insertion 1, Christopher B Wilson
MGI accession ID: MGI:2386448
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: CD4^Cre; CD4cre; CD4-Cre; CD4-Cre⁺; CD4-cre^Tg; CD4p-Cre; Tg(CD4-cre)^1Cw1
Marker symbol: Tg(Cd4-cre)1Cwi
Marker name: transgene insertion 1, Christopher B Wilson
Marker synonyms: CD4cre; CD4-Cre; CD4-Cre⁺; CD4-cre^Tg; CD4p-Cre; Tg(CD4-cre)1Cwi
Chromosome: 3
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: T cells
Molecular note: Cre recombinase is expressed under the control of mouse Cd4 regulatory elements. The Cd4 enhancer, promoter and silencer collectively drive expression at sequential stages specifically of CD4⁺ T cell development. The integration site of the transgene construct is at position 60335693-60341285 (qD) of chromosome 3 and the insert contains at least 15 complete sequential copies of the transgenic construct.