Atp2b4 or PMCA4 (Ca++ transporting ATPase, plasma membrane 4) encodes a P-type primary ion transport ATPase. This ATPase is involved in intracellular calcium homeostasis and is expressed in aorta, portal vein, bladder, diaphragm, seminal vesicles, and testis. 
Mice homozygous for this null allele are viable, however, male mice are infertile. Male infertility is due to loss of the the major plasma membrane Ca-ATPase (PMCA) of the sperm tail, resulting in the inability of sperm to achieve the hyperactivated motility needed for fertilization. On a mixed 129 and Black Swiss background, portal vein smooth muscle cells from homozygous mice exhibit increased apoptosis and reduced phasic contractions. Mice on the mixed background develop enlarged submandibular glands, skin irritation, accelerated osteoclastogenesis, osteopenia with an increase in osteoclasts on bone surface, and attenuated Ca signaling in B lymphocytes. On a congenic FVB background, homozygotes exhibit increased cardiac hypertrophy following pressure overload stimulation.



This strain may be useful for studying calcium signaling in male infertility, lymphocytes, osteoclasts and smooth muscle contractility.

Mice homozygous for a null Atp2b4 or PMCA4 (Ca++ transporting ATPase, plasma membrane 4) allele exhibit male infertility (due to loss of hyperactivate sperm motility), increased apoptosis in portal vein smooth muscle cells, accelerated osteoclastogenesis, osteopenia, attenuated Ca signaling in B lymphocytes,and increased cardiac hypertrophy following pressure overload stimulation.
This strain may be useful for studying calcium signaling in male infertility, lymphocytes, osteoclasts and smooth muscle contractility.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.