Mice homozygous for a null Atp2b4 or PMCA4 (Ca++ transporting ATPase, plasma membrane 4) allele exhibit male infertility (due to loss of hyperactivate sperm motility), increased apoptosis in portal vein smooth muscle cells, accelerated osteoclastogenesis, osteopenia, attenuated Ca signaling in B lymphocytes,and increased cardiac hypertrophy following pressure overload stimulation. This strain may be useful for studying calcium signaling in male infertility, lymphocytes, osteoclasts and smooth muscle contractility.
Gary E Shull, University of Cincinnati
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Atp2b4 | ATPase, Ca++ transporting, plasma membrane 4 |
A targeting vector containing the neomycin resistance gene was used to replace codons 448-474 in exon 11, a region encoding the catalytic phosphorylation site. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to Black Swiss mice. Mice from the colony were then backcrossed to FVB/N for at least 10 generations. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
Allele Name | targeted mutation 1, Gary E Shull |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Pmca4- |
Gene Symbol and Name | Atp2b4, ATPase, Ca++ transporting, plasma membrane 4 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 1 |
Molecular Note | A region of exon 11 encoding amino acids 448-474, including the catalytic phosphorylation site (aspartic acid 465), was replaced with a neomycin resistance gene. Northern blot showed no reduction in mRNA levels in mutant mice. Exon-11-deficient mice express a mutant PMCA4 mRNA as a consequence of exon-10 splicing to exon-12, which preserves the reading frame and enables these mice to produce functionless mutant PMCA4a and PMCA4b proteins (devoid of a catalytic site). |
While maintaining a live colony, these mice are bred as heterozygotes or homozygous female x heterozygous male. Male mice homozygous for the mutation are infertile.
When using the FVB.129(Cg)-Atp2b4tm1Ges/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36807 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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