These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4 of the nuclear GTPase, germinal center associated (Nuggc) gene. This strain may be useful for studying the increase in G:C transitions in B lymphocytes.
Marilyn Diaz, NIEHS
These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4, including the highly conserved GTPase domain, of the nuclear GTPase, germinal center associated (Nuggc) gene. Nuggc is expressed in germinal center B cell lymphocytes and may protect B cells from activation-induced deaminase (AID)-dependent DNA breaks. Nuggc is highly expressed in germinal center-derived B cell lymphomas. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-4 deleted in cre-expressing tissues.
For example, when crossed to Sox2-cre mice, expressing embryonic Cre recombinase in (see Stock No. 008454), Nuggc deficiency in offspring results in an increase in G:C transitions in B cells from Peyer's Patches.
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 4 of the nuclear GTPase, germinal center associated (Nuggc) gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 2-4, intact floxed-neo cassette, or excision of both exons 2-4 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exons 2-4, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Progeny were crossed to remove the Cre transgene and these Slip-GC were bred to C57BL/6J mice for at least 6 generations. Mice were then bred to BALB/c mice for at least 6 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.2, Marilyn Diaz|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Nuggc, nuclear GTPase, germinal center associated|
|Strain of Origin||C57BL/6|
|Molecular Note||A loxP site was inserted upstream of exon 2. A floxed neo cassette was inserted downstream of exon 4. Cre-mediated recombination removed the neo cassette and left exons 2 through 4 floxed.|
When maintaining a live colony, mice homozygous for the floxed allele may be bred together.
When using the C.B6-Nuggctm1.2Diaz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022017 in your Materials and Methods section.