These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4 of the nuclear GTPase, germinal center associated (Nuggc) gene. This strain may be useful for studying the increase in G:C transitions in B lymphocytes.
Marilyn Diaz, NIEHSRead More +
These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4, including the highly conserved GTPase domain, of the nuclear GTPase, germinal center associated (Nuggc) gene. Nuggc is expressed in germinal center B cell lymphocytes and may protect B cells from activation-induced deaminase (AID)-dependent DNA breaks. Nuggc is highly expressed in germinal center-derived B cell lymphomas. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-4 deleted in cre-expressing tissues.
For example, when crossed to Sox2-cre mice, expressing embryonic Cre recombinase in (see Stock No. 008454), Nuggc deficiency in offspring results in an increase in G:C transitions in B cells from Peyer's Patches.
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 4 of the nuclear GTPase, germinal center associated (Nuggc) gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 2-4, intact floxed-neo cassette, or excision of both exons 2-4 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exons 2-4, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Progeny were crossed to remove the Cre transgene and these Slip-GC were bred to C57BL/6J mice for at least 6 generations. Mice were then bred to BALB/c mice for at least 6 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.2, Marilyn Diaz|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Nuggc, nuclear GTPase, germinal center associated|
|Gene Synonym(s)||C8orf80; Gm600; Gm600; HMFN0672; LOC239151; RGD1562099; SLIP-GC; gene model 600, (NCBI); predicted gene 600|
|Strain of Origin||C57BL/6|
|Molecular Note||A loxP site was inserted upstream of exon 2. A floxed neo cassette was inserted downstream of exon 4. Cre-mediated recombination removed the neo cassette and left exons 2 through 4 floxed.|
When maintaining a live colony, mice homozygous for the floxed allele may be bred together.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
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