These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4 of the nuclear GTPase, germinal center associated (Nuggc) gene. This strain may be useful for studying the increase in G:C transitions in B lymphocytes.
Marilyn Diaz, NIEHS
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Nuggc | nuclear GTPase, germinal center associated |
These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4, including the highly conserved GTPase domain, of the nuclear GTPase, germinal center associated (Nuggc) gene. Nuggc is expressed in germinal center B cell lymphocytes and may protect B cells from activation-induced deaminase (AID)-dependent DNA breaks. Nuggc is highly expressed in germinal center-derived B cell lymphomas. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-4 deleted in cre-expressing tissues.
For example, when crossed to Sox2-cre mice, expressing embryonic Cre recombinase in (see Stock No. 008454), Nuggc deficiency in offspring results in an increase in G:C transitions in B cells from Peyer's Patches.
A targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 4 of the nuclear GTPase, germinal center associated (Nuggc) gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 2-4, intact floxed-neo cassette, or excision of both exons 2-4 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exons 2-4, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Progeny were crossed to remove the Cre transgene and these Slip-GC were bred to C57BL/6J mice for at least 6 generations. Mice were then bred to BALB/c mice for at least 6 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.2, Marilyn Diaz |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Nuggc, nuclear GTPase, germinal center associated |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 14 |
Molecular Note | A loxP site was inserted upstream of exon 2. A floxed neo cassette was inserted downstream of exon 4. Cre-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. |
When maintaining a live colony, mice homozygous for the floxed allele may be bred together.
When using the C.B6-Nuggctm1.2Diaz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #022017 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Nuggc<tm1.2Diaz> |
Frozen Mouse Embryo | C.B6-Nuggc<tm1.2Diaz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C.B6-Nuggc<tm1.2Diaz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C.B6-Nuggc<tm1.2Diaz>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C.B6-Nuggc<tm1.2Diaz>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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