These Utxflox mice possess loxP sites flanking exon 3 of the Kdm6a (lysine (K)-specific demethylase 6A) gene and have applications in studies related to histone methylation and transcriptional regulation during development.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
The Kdm6a (lysine (K)-specific demethylase 6A) gene encodes a histone demethylase that acts on lysine 27 of histone H3, and is involved in regulation of embryogenesis via the WNT signaling pathway. These mice possess loxP sites on either side of exon 3 of the targeted gene. Homozygous females and hemizygous males are viable and fertile (the gene is X linked). When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
A targeting vector, obtained from EUCOMM, containing a FRT site flanked beta-geo cassette and a loxP site was inserted upstream of exon 3. A second loxP site was inserted downstream of exon 3. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived v6.5 embryonic stem (ES) cells, which were transiently transfected with a FLP recombinase vector to remove the selection cassette. ES cells that had successfully undergone FLP-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon 3 were injected into B6D2F2 blastocysts. The resulting male chimeric animals were bred to C57BL/6 female mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony. The gene is X linked.
|Allele Name||targeted mutation 1c, Rudolf Jaenisch|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Kdm6a, lysine (K)-specific demethylase 6A|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||A targeting vector, obtained from EUCOMM, containing a FRT site flanked beta-geo cassette and a loxP site was inserted upstream of exon 3. A second loxP site was inserted downstream of exon 3. ES cells were transiently transfected with a FLP recombinase vector to remove the selection cassette.|
When maintaining a live colony, these mice can be bred as homozygous females and hemizygous males (the gene is X linked).
When using the Utxflx mouse strain in a publication, please cite the originating article(s) and include JAX stock #021926 in your Materials and Methods section.