These Utxflox mice possess loxP sites flanking exon 3 of the Kdm6a (lysine (K)-specific demethylase 6A) gene and have applications in studies related to histone methylation and transcriptional regulation during development.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Kdm6a | lysine (K)-specific demethylase 6A |
The Kdm6a (lysine (K)-specific demethylase 6A) gene encodes a histone demethylase that acts on lysine 27 of histone H3, and is involved in regulation of embryogenesis via the WNT signaling pathway. These mice possess loxP sites on either side of exon 3 of the targeted gene. Homozygous females and hemizygous males are viable and fertile (the gene is X linked). When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
A targeting vector, obtained from EUCOMM, containing a FRT site flanked beta-geo cassette and a loxP site was inserted upstream of exon 3. A second loxP site was inserted downstream of exon 3. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived v6.5 embryonic stem (ES) cells, which were transiently transfected with a FLP recombinase vector to remove the selection cassette. ES cells that had successfully undergone FLP-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exon 3 were injected into B6D2F2 blastocysts. The resulting male chimeric animals were bred to C57BL/6 female mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony. The gene is X linked.
Allele Name | targeted mutation 1c, Rudolf Jaenisch |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | Utxflx |
Gene Symbol and Name | Kdm6a, lysine (K)-specific demethylase 6A |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | X |
Molecular Note | A targeting vector, obtained from EUCOMM, containing a FRT site flanked beta-geo cassette and a loxP site was inserted upstream of exon 3. A second loxP site was inserted downstream of exon 3. ES cells were transiently transfected with a FLP recombinase vector to remove the selection cassette. |
When maintaining a live colony, these mice can be bred as homozygous females and hemizygous males (the gene is X linked).
When using the Utxflx mouse strain in a publication, please cite the originating article(s) and include JAX stock #021926 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked -Heterozygous females and Wild-type males for Kdm6a<tm1c(EUCOMM)Jae> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.