Overview

Also Known As:BXSB.Ighm-/-, BXSB.Yaa Ighm-/-, Ighm-deficient BXSB.Yaa, BXSB.Ighm null, BXSB.Cmu-, BXSB.muMT-, BXSB.IgM-

Ighm-deficient BXSB.Yaa mice (BXSB.Ighm-/- or BXSB.Yaa Ighm-/-) are a BXSB/MpJ-congenic strain carrying a null mutation of the immunoglobulin heavy chain of the class mu. The mature B cell-deficiency of BXSB.Ighm-/- mice prevents manifestation of the spontaneous lupus-like autoimmune syndrome observed for BXSB/MpJ inbred mice. These BXSB.Ighm-/- mice may be useful in studying the role of B cells in autoimmune disease, and other mechanisms that are not B cell-dependent (e.g., dendritic cells and T cells).

Donating Investigator

Derry Roopenian, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Ighm^tm1Cgn

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Ighm	immunoglobulin heavy constant mu

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Research Applications

Research Tools
Cell Biology Research
Immunology, Inflammation and Autoimmunity Research
Hematological Research
Developmental Biology Research
Mouse/Human Gene Homologs
Internal/Organ Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Ighm-deficient BXSB.Yaa mice (BXSB.Ighm-/- or BXSB.Yaa Ighm-/-) are a BXSB/MpJ-congenic strain carrying a null mutation of the immunoglobulin heavy chain of the class mu.

BXSB/MpJ inbred males (Stock No. 000740) develop a spontaneous lupus-like autoimmune syndrome: mortality starts at ~13 weeks of age with 50% lethality by ~30 weeks and 76% lethality by ~40 weeks. BXSB/MpJ inbred females develop a greatly attenuated form of autoimmune disease because they lack Yaa.

Homozygous (Ighm-/-) mice are viable and fertile. Homozygotes lack mature B cells and have no expression of membrane-bound IgM; although some B cells may be produced using immunoglobulin genes other than mu. As such, they should be maintained in pathogen-free conditions on sulfatrim water to increase colony health (similar to other immunodeficient strains). Compared to BXSB/MpJ, the mature B cell-deficiency of BXSB.Ighm-/- mice prevents manifestation of the spontaneous autoimmune phenotype in both males and females.

Heterozygous males (BXSB.Ighm+/-) develop the BXSB/MpJ autoimmune phenotype. Heterozygous females develop a greatly attenuated form of autoimmune disease because they lack Yaa.

C57BL/6J-congenic mice harboring this Ighm null allele are described and available from The Jackson Laboratory Repository as Stock No. 002288.

Development

The Ighm null allele (Cμ-, μMT-, IgM-, or Ighm^tm1Cgn) was designed by Dr. Klaus Rajewsky (University of Cologne) with a neomycin resistance cassette disrupting one of the membrane exons of the Ighm gene (immunoglobulin heavy constant mu) on chromosome 12. C57BL/6J-congenic mice harboring this Ighm null allele are described and available from The Jackson Laboratory Repository as Stock No. 002288.
Dr. Derry C. Roopenian (The Jackson Laboratory) obtained some of these mutant mice and backcrossed them with BXSB/MpJ inbred mice (Stock No. 000740) for 12 generations, and then maintained the colony by breeding homozygous mice together. In 2013, Dr. Roopenian sent some BXSB.Yaa Ighm-/- mice at generation N12F12 to The Jackson Laboratory Repository (Autoimmune Resource) to establish Stock No. 021871. Male mice have the BXSB/MpJ-derived Y chromosome that contains the Y-linked autoimmune accelerator locus (Yaa).

Control Suggestions

000740 BXSB/MpJ

Additional Information

Considerations for Choosing Controls

Selected References

McPhee CG; Bubier JA; Sproule TJ; Park G; Steinbuck MP; Schott WH; Christianson GJ; Morse HC 3rd; Roopenian DC. 2013. IL-21 is a double-edged sword in the systemic lupus erythematosus-like disease of BXSB.Yaa mice. J Immunol 191(9):4581-8PubMed: 24078696 MGI: J:206236

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Genetics

Ighm^tm1Cgn

Allele Symbol: Ighm^tm1Cgn

Allele Name	targeted mutation 1, University of Cologne
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	B cell^-; BCKO; BCR-; BKO; Cmu ^-; Ig- muMT; Ig^-; IgH-; IgH^muMT; Igh-6-; Igh-6^null; Igh-6^tm1Cgn; Igh-6tm1CgnmuMT⁰; Ighm^-; Igmu^null; muIgKO; mum^-; muMT; muMt-; mu-MT^-
Gene Symbol and Name	Ighm, immunoglobulin heavy constant mu
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	12
Molecular Note	A neomycin resistance cassette disrupted one of the membrane exons of the gene encoding immunoglobulin heavy chain of the class mu (IgM).
Mutations Made By	Daisuke Kitamura, University of Cologne

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ighm^tm1Cgn related

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B cell deficiency
- Cancer Research
  - B cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B cell deficiency

Cell Biology Research
- Signal Transduction
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin
  - B cell deficiency
  - B cell specific marker
  - B cell specific surface marker
  - production of B cells and antibodies
- Genetics Research
  - Tissue/Cell Markers: B cell specific marker
- Hematological Research
Immunology, Inflammation and Autoimmunity Research
- Intracellular Signaling Molecules
- Immunodeficiency
  - defects in humoral immune responses
  - B cell defects
  - B cell deficiency
- Autoimmunity
  - B cell deficiency
  - lupus erythematosus, control
  - lupus erythematosus
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
- Lymphoid Tissue Defects
  - hematopoietic development
- Growth Factors/Receptors/Cytokines
Hematological Research
- Hematopoietic Defects
Developmental Biology Research
- Internal/Organ Defects
  - Lymphoid Tissue Defects
  - hematopoietic defects
- Lymphoid Tissue Defects
  - hematopoietic defects
Mouse/Human Gene Homologs
- systemic lupus erythematosus
Internal/Organ Research
- Lymphoid Tissue Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Ighm^tm1Cgn/Ighm^tm1Cgn X/Yaa
BXSB.129S2(B6)-Ighm/Dcr

immune system phenotype

decreased susceptibility to systemic lupus erythematosus
- this null allele diminishes the B cells essential for the Yaa-induced development of Lupus-like autoimmune disease such that these males survive beyond 40 weeks, and do not have the increased splenic populations of CD4+ T cells or CD11b+ monocytes, nor is there elevated ICOS expression in splenic T cells

References

McPhee CG; Bubier JA; Sproule TJ; Park G; Steinbuck MP; Schott WH; Christianson GJ; Morse HC 3rd; Roopenian DC. 2013. IL-21 is a double-edged sword in the systemic lupus erythematosus-like disease of BXSB.Yaa mice. J Immunol 191(9):4581-8PubMed: 24078696 MGI: J:206236

Additional - Ighm^tm1Cgn related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Ighm
- Genotyping resources and troubleshooting
Breeding Considerations

To maintain the live colony, homozygous mice may be bred together. Homozygous animals of both sexes do not develop the spontaneous lupus-like autoimmune syndrome observed for BXSB/MpJ inbred mice (Stock No. 000740). Homozygous mice are B cell-deficient. As such, they should be maintained in pathogen-free conditions on sulfatrim water to increase colony health (similar to other immunodeficient strains). Heterozygotes will develop the sex-specific autoimmune phenotype of BXSB/MpJ. The expected coat color is white-bellied agouti.
- Additional Breeding and Husbandry Support
Citation
When using the BXSB.129S2(B6)-Ighm^tm1Cgn/DcrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #021871 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous for Ighm<tm1Cgn>

Related Products and Services

Frozen Mouse Embryo	BXSB.129S2(B6)-Ighm<tm1Cgn>/DcrJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	BXSB.129S2(B6)-Ighm<tm1Cgn>/DcrJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	BXSB.129S2(B6)-Ighm<tm1Cgn>/DcrJ Frozen Embryos	$3373.50
Frozen Mouse Embryo	BXSB.129S2(B6)-Ighm<tm1Cgn>/DcrJ Frozen Embryos	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:BXSB.Ighm-/-, BXSB.Yaa Ighm-/-, Ighm-deficient BXSB.Yaa, BXSB.Ighm null, BXSB.Cmu-, BXSB.muMT-, BXSB.IgM-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Ighmtm1Cgn

Allele Symbol: Ighmtm1Cgn

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Ighmtm1Cgn related

Mammalian Phenotype Terms by Genotype

Genotype: Ighmtm1Cgn/Ighmtm1Cgn X/YaaBXSB.129S2(B6)-Ighm/Dcr

immune system phenotype

References

Additional - Ighmtm1Cgn related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Ighm^tm1Cgn

Allele Symbol: Ighm^tm1Cgn

Genotype: Ighm^tm1Cgn related

Genotype: Ighm^tm1Cgn/Ighm^tm1Cgn X/Yaa
BXSB.129S2(B6)-Ighm/Dcr

Additional - Ighm^tm1Cgn related