This mCherry-LC3 transgenic line expresses a mCherry-Map1lc3a, Red Fluorescent protein- microtubule-associated protein 1 light chain 3 alpha fusion gene under the direction of a mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter. Applications exist in studies related to cardiac autophagy.
Roberta Gottlieb, Cedars-Sinai Heart Institute
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Autophagy, the highly conserved catabolic mechanism, employs the lysosome system to break down and recycle aged and damaged cellular components, such as macromolecules and organelles. It is a physiological adaptation strategy in response to stress and starvation, as well as an immune mechanism during infection. The processed isoform encoded by Map1lc3a (also called LC3) binds to autophagosomal membranes.
These mCherry-LC3 transgenic mice express mCherry-Map1lc3a (Red Fluorescent protein- microtubule-associated protein 1 light chain 3 alpha) fusion gene, under the control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Transgene expression is detected in autophagosomes of myocardiocytes. Rapamycin administration to transgenic mice increases the number of fluorescently labeled autophagosomes in the myocardium. Coadministration of chloroquine with rapamycin further increases the number of autophagosomes. Monodansylcadaverine labeling co-localizes with mCherry fluorescence. The Donating Investigator reports that transgene expression level has been stable since the line was established. Mice that are homozygous for the targeted mutation are viable but express high levels of the transgene fusion protein, which may form protein aggregates and/or dysregulate autophagy. Hemizygotes are viable and fertile.
A transgenic insert designed by Dr. Roberta A. Gottlieb (San Diego State University) containing an mCherry-Map1lc3a, Red Fluorescent protein- microtubule-associated protein 1 light chain 3 alpha fusion gene, under the control of the mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter was injected into fertilized FVB/N mouse eggs. The founder line was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Expressed Gene | Map1lc3a, microtubule-associated protein 1 light chain 3 alpha, mouse, laboratory |
Site of Expression | Transgene expression is detected in autophagosomes of myocardiocytes. |
Allele Name | transgene insertion 1, Roberta Gottlieb |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | mCherry-LC3 |
Gene Symbol and Name | Tg(Myh6-mCherry/Map1lc3a)1Rag, transgene insertion 1, Roberta Gottlieb |
Gene Synonym(s) | |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | Map1lc3a, microtubule-associated protein 1 light chain 3 alpha, mouse, laboratory |
Site of Expression | Transgene expression is detected in autophagosomes of myocardiocytes. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic insert contains an mCherry-Map1lc3a, Red Fluorescent protein- microtubule-associated protein 1 light chain 3 alpha fusion gene, under the control of the mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter. Line 1 was generated. |
When maintaining a live colony, hemizygous or homozygous mice may be bred together, to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator recommends using hemizygotes for research as mice homozygous for the transgene express high levels of the fusion protein, which may form protein aggregates and/or dysregulate autophagy.
When using the FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021853 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Myh6-mCherry/Map1lc3a)1Rag |
Frozen Mouse Embryo | FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J | $3373.50 |
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