These loxP-flanked knockin mice carry an amino acid substitution, R1207H in exon 24, as well as loxP sites flanking exons 24 to 28 of the Eif4g1 gene. Mutations in Eif4g1, a translation initiation factor, are associated with susceptibility to Parkinson's disease.
Kuldip Dave, The Michael J. Fox Foundation
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Humanized sequence) | Eif4g1 | eukaryotic translation initiation factor 4, gamma 1 |
Eif4g1 or eukaryotic translation initiation factor 4, gamma 1 is one of a complex of initiation factors associated with mRNA posttranscriptional modification.
An amino acid substitution, R1205H, found in Eif4g1 is associated with susceptibility to Parkinson's in humans. This conditional knockin strain carries the corresponding mutation in the mouse, R1207H in exon 24, as well as loxP sites flanking exons 24 to 28. Homozygous mice are viable and fertile and express the R1207H mutation. Cre-mediated recombination removes exons 24-28 resulting in a null allele. This strain may be useful for studying Parkinson's disease.
The targeting vector was designed (by site-directed mutagenesis) to insert the arginine to histidine amino acid substitution at position 1207 (R1207H) (human 1205) into exon 24 and place a loxP site upstream of exon 24, followed by an FRT-flanked neomycin cassette and a loxP site downstream of exon 28. The construct was electroporated into C57BL/6 derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to FlpE-deleter mice on a C57BL/6 genetic background, to remove the neomycin cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, The Michael J Fox Foundation |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), Humanized sequence) |
Allele Synonym(s) | Eif4g1R1207H |
Gene Symbol and Name | Eif4g1, eukaryotic translation initiation factor 4, gamma 1 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 16 |
Molecular Note | The targeting vector was designed (by site-directed mutagenesis) to insert the arginine to histidine amino acid substitution at position 1207 (R1207H) (human 1205) into exon 24 and place a loxP site upstream of exon 24, followed by an FRT-flanked neomycin cassette and a loxP site downstream of exon 28. Flp-mediated recombination removed the neomycin cassette. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Eif4g1R1207H mouse strain in a publication, please cite the originating article(s) and include JAX stock #021827 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Eif4g1<tm1.1Mjff> |
Frozen Mouse Embryo | B6(Cg)-Eif4g1<tm1.1Mjff>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Eif4g1<tm1.1Mjff>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Eif4g1<tm1.1Mjff>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Eif4g1<tm1.1Mjff>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.