These H3f3bc/c mutant mice possess loxP sites flanking a wildtype H3 histone isoform 3 (H3.3) coding sequence and STOP sequence. When bred to mice expressing cre-recombinase, removal of the floxed sequence results in CFP expression. This strain may be useful for studying the role of histone H3.3 during development.
Jeff Mann, Murdoch Childrens Research Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | H3f3b | H3 histone, family 3B |
The H3f3bc/c targeting vector contains, from 5' to 3', a synthetic splice acceptor, a loxP-site, a wildtype H3 histone isoform 3 (H3.3) coding sequence, H3f3b (H3 histone, family 3B) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a second loxP site, an ATG start codon, the cerulean variant of cyan fluorescent protein (CFP), and another SV40 polyA sequence. This vector replaced all of exon 2 of the H3f3b gene. H3f3b encodes the histone H3 isoform, H3.3, involved in the regulation of chromatin activity during DNA replication. Homozygous H3f3bc/c mice are viable and fertile, and express wildtype H3f3b. In the absence of Cre, CFP expression is prevented by the H3.3 coding sequence. When bred to mice that express Cre recombinase, resulting offspring will have the wildtype loxP-flanked H3.3 coding sequence deleted in the cre-expressing tissues, allowing CFP to be expressed.
A targeting vector was designed to contain, from 5' to 3', a synthetic splice acceptor, a loxP site, an ATG start codon, a wildtype H3 histone isoform 3 (H3.3) coding sequence, H3f3b (H3 histone, family 3B) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a downstream frt-flanked neomycin resistance (neo) cassette, and a second loxP site, an ATG start codon, the cerulean variant of cyan fluorescent protein (CFP), and another SV40 polyA sequence. This vector replaced all of exon 2 of the H3f3b gene. The construct was electroporated into 129S1/SvImJ-derived A2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into (C57BL/6 J x CBA/CaH) blastocysts and resulting chimeric mice were bred with 129S1;129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice to delete the neo cassette. Resulting offspring were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the conditional-H3f3b (H3f3bc/c) allele. These mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least 5 generations. Upon arrival, mice were bred to 129S1/SvImJ inbred mice for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Jeff Mann |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | H3f3bc |
Gene Symbol and Name | H3f3b, H3 histone, family 3B |
Gene Synonym(s) | |
Strain of Origin | 129S1/SvImJ |
Chromosome | 11 |
Molecular Note | A targeting vector was designed to contain, from 5' to 3', a synthetic splice acceptor, a loxP site, an ATG start codon, a wildtype H3 histone isoform 3 (H3.3) coding sequence, H3f3b (H3 histone, family 3B) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a downstream frt-flanked neomycin resistance (neo) cassette, and a second loxP site, an ATG start codon, the cerulean variant of cyan fluorescent protein (CFP), and another SV40 polyA sequence. This vector replaced all of exon 2. Flp-mediated recombination removed the neo cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the 129S-H3f3btm1.1Mnn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021789 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for H3f3b<tm1.1Mnn> |
Frozen Mouse Embryo | 129S-H3f3b<tm1.1Mnn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S-H3f3b<tm1.1Mnn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129S-H3f3b<tm1.1Mnn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129S-H3f3b<tm1.1Mnn>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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