The last coding exon (exon 24) of the Nrxn1 (neurexin 1) gene is flanked by loxP sites in this conditional mutant allele. Cre excision of the floxed segment enables the production of truncated protein. A 2xHA (hemagglutinin) tag outside of the floxed region provides a useful marker.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Nrxn1 | neurexin I |
Neurexins act as cell adhesion molecules and receptors involved with synaptic function. In this conditional targeted mutation strain, the last coding exon (exon 24) of the Nrxn1 (neurexin 1) gene is flanked by loxP sites. Excision of the floxed segment through Cre recombinase expression effectively truncates the gene product. A 2xHA (hemagglutinin) tag outside of the floxed region provides a useful marker. Immunoblotting, immunohistochemistry, and immunocytochemistry confirm that the HA tag is functional.
Exon 24, the last coding exon of the gene, encodes the transmembrane region of the protein as well as the cytoplasmic C-terminal. A 2xHA (hemagglutinin) tag followed by a loxP site was inserted in-frame into exon 24. A second loxP site was placed after the stop codon, immediately followed by an FRT-flanked PGK-neomycin resistance cassette. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 five times by the donating laboratory.
Allele Name | targeted mutation 3, Thomas C Sudhof |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | LV1-Nrxn1-HA-tagged |
Gene Symbol and Name | Nrxn1, neurexin I |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 17 |
Molecular Note | Exon 24, the last coding exon of the gene, encodes the transmembrane region of the protein as well as the cytoplasmic C-terminal. A 2xHA (hemagglutinin) tag followed by a loxP site was inserted in-frame into exon 24. A second loxP site was placed after the stop codon, immediately followed by an FRT-flanked PGK-neomycin resistance cassette. |
Homozygous and heterozygous mice are viable and fertile.
When using the B6.129-Nrxn1tm3Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021777 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Nrxn1<tm3Sud> |
Frozen Mouse Embryo | B6.129-Nrxn1<tm3Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Nrxn1<tm3Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Nrxn1<tm3Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Nrxn1<tm3Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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