These mice carry a knockout allele of Glg1 (golgi apparatus protein 1; also known as E-selectin ligand 1) which may be useful in studies of skeletal growth and homeostasis.
Brendan Lee, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Glg1 | golgi apparatus protein 1 |
The majority of human skeletal dysplasias are caused by dysregulation of growth plate homeostasis. Glg1 (golgi apparatus protein 1; also known as E-selectin ligand 1) regulates growth plate homeostasis in mice by inhibiting the intracellular processing and secretion of mature transforming growth factor beta (TGFB).
These mice carry a knockout allele of Glg1 which involves a deletion of exons 13-16. Homozygous embryos and neonates (E14.5, E17.5, P3, and P7) are smaller than wildtype littermates. At birth, heterozygotes appear normal, but homozygous knockout pups are approximately 30%-50% smaller and show a higher rate of perinatal lethality.
Skeletal preparations of homozygotes show generalized shortening and thinning of all bony elements and distinctively narrow rib cages. A shortening of growth plates affecting both the proliferating zone (PZ) and the hypertrophic zone (HZ) involves decreased chondrocyte cell density and proliferation as well as increased extracellular matrix deposition. This is accompanied by increased mature TGFB2 and phosphorylated SMAD2 in the cartilage.
Exons 13-16 were replaced with a puromycin resistance cassette and 3' Hprt minigene in the AB2.2 129S7/SvEvBrd-Hprt1+-derived embryonic stem (ES) cell line. Resultant chimeric males were mated with C57BL/6J females. This strain was maintained on a mixed C57BL6 and 129 genetic background by the donating laboratory.
Allele Name | targeted mutation 1, Brendan Lee |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Glg1, golgi apparatus protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 8 |
Molecular Note | Exons 13 through 16 were replaced with a puro cassette and 3' Hprt minigene. The absence of protein expression was confirmed by western blot on brain extracts. |
Heterozygotes are viable and fertile. Homozygotes show a higher rate of perinatal lethality and are infertile.
When using the B6;129S7-Glg1tm1Brle/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021770 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for |
Frozen Mouse Embryo | B6;129S7-Glg1<tm1Brle>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Glg1<tm1Brle>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Glg1<tm1Brle>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Glg1<tm1Brle>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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