These Gakflox mutant mice possess loxP sites flanking exon 1 of the cyclin G associated kinase (Gak) gene. GAK, a cyclin-dependent protein kinase, is a multidomain protein that functions as a cochaperone with Hspa8 (Hsc70). GAK is involved in the process of clathrin-mediated endocytosis and is critical to development. 
Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have a null allele in the cre-expressing tissues. 

For example, when bred to mice carrying Tg(Nes-Cre)1Kln, neuronal Cre recombinase expression results in early lethality and a failure of neuroprogenitor cells to proliferate.

When bred to mice carrying Tg(Alb-cre)21Mgn, Cre recombinase expression in the liver results in lethality by day 5, jaundice, disorganized bile ducts, undifferentiated hepatoblasts, fragmented Golgi and failure to accumulate glycogen. 


When bred to adult mice carrying Tg(CAG-cre/Esr1*)5Amc, tamoxifen-inducible cre-recombinase expression results in death within days of tamoxifen treatment.


When bred to mice carrying Tg(KRT14-Cre)1Amc, Cre recombinase expression results in the epidermis, results in postnatal lethality, a defective epidermal barrier and failure to differentiate epidermal layers.

These Gakflox mutant mice possess loxP sites flanking exon 1 of the cyclin G associated kinase (Gak) gene. GAK functions as a cochaperone with Hspa8 (Hsc70). GAK is involved in the process of clathrin-mediated endocytosis and is critical to development.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.