These Gakflox mutant mice possess loxP sites flanking exon 1 of the cyclin G associated kinase (Gak) gene. GAK functions as a cochaperone with Hspa8 (Hsc70). GAK is involved in the process of clathrin-mediated endocytosis and is critical to development.
Lois Greene, NIH
These Gakflox mutant mice possess loxP sites flanking exon 1 of the cyclin G associated kinase (Gak) gene. GAK, a cyclin-dependent protein kinase, is a multidomain protein that functions as a cochaperone with Hspa8 (Hsc70). GAK is involved in the process of clathrin-mediated endocytosis and is critical to development.
Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have a null allele in the cre-expressing tissues.
For example, when bred to mice carrying Tg(Nes-Cre)1Kln, neuronal Cre recombinase expression results in early lethality and a failure of neuroprogenitor cells to proliferate.
When bred to mice carrying Tg(Alb-cre)21Mgn, Cre recombinase expression in the liver results in lethality by day 5, jaundice, disorganized bile ducts, undifferentiated hepatoblasts, fragmented Golgi and failure to accumulate glycogen.
When bred to adult mice carrying Tg(CAG-cre/Esr1*)5Amc, tamoxifen-inducible cre-recombinase expression results in death within days of tamoxifen treatment.
When bred to mice carrying Tg(KRT14-Cre)1Amc, Cre recombinase expression results in the epidermis, results in postnatal lethality, a defective epidermal barrier and failure to differentiate epidermal layers.
The targeting vector contains a loxP site followed by exon 1 and loxP-flanked PGK neomycin cassette. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice once and then maintained by sibling mating.
|Allele Name||targeted mutation 2, Lois E Greene|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Gak, cyclin G associated kinase|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A loxP site was inserted upstream of exon 1. A floxed neo cassette was inserted downstream of exon 1.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129S6-Gaktm2Legr/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36793 in your Materials and Methods section.