The α-MHC-mCherry transgene has a modified mouse alpha myosin heavy-chain (Myh6) promoter sequence directing cardiac-specific mCherry expression in cardiomyocytes. These αMHC-mCherry transgenic mice may be useful in studying cardiac regeneration/myogenesis following infarction.
Michael I Kotlikoff, Cornell University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
The α-MHC-mCherry transgene has a modified mouse alpha myosin heavy-chain (Myh6) promoter sequence directing cardiac-specific mCherry expression in cardiomyocytes. mCherry expression may be observed in cardiomyocytes by direct/native fluorescence. The donating investigator reports that mCherry fluorescence is very strong and is visible through the chest wall of neonatal mice. The donating investigator reports that homozygous mice are viable and fertile, with no reported phenotypic abnormalities.
Of note, the same donating investigator has several strains at The Jackson Laboratory Repository; including these αMHC-mCherry transgenic mice (Stock No. 021577), the smooth-muscle tool strain smMHC/Cre/eGFP (Stock No. 007742),
the cholinergic/neuromuscular tool strain ChAT(BAC)-eGFP (Stock No. 007902),
and the conditional cardiac-expressing eGFP-calmodulin fusion protein strain ccGCaMP2 (Stock No. 012477).
The α-MHC-mCherry transgene contains a cardiac-specific alpha myosin heavy-chain (Myh6) mouse promoter sequence upstream of an mCherry fluorescent protein cDNA sequence. The modified mouse Myh6 promoter sequence is minimally active/attenuated through the ablation of three GATA sites and two thyroid-like response elements, thereby directing cardiac-specific mCherry expression and decreased background expression. The ~6.9 kbp α-MHC-mCherry transgene was injected into (C57BL/6J x DBA/2NCrl)F2 mouse oocytes. Founder mice were bred with C57BL/6J mice to establish the colony. αMHC-mCherry transgenic mice from founder line 2 were found to have extremely bright mCherry fluorescence in the heart at all stages of development. This founder line was subsequently bred to C57BL/6J mice for a total of two generations. Transgenic mice were then bred together for at least seven generations prior to sending to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. To generate the living colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J female mice (Stock No. 000664). The αMHC-mCherry transgenic mice were then bred together, to wildtype mice from the colony, or to C57BL/6J mice.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
---|---|
Site of Expression | mCherry expression is observed in cardiomyocytes. |
Allele Name | transgene insertion 2, Michael I Kotlikoff |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | alpha-MHC-mCherry; alphaMHC-mCherry; a-MHC-mCherry; aMHC-mCherry; cardiac mCherry |
Gene Symbol and Name | Tg(Myh6*-mCherry)2Mik, transgene insertion 2, Michael I Kotlikoff |
Gene Synonym(s) | |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | mCherry expression is observed in cardiomyocytes. |
Strain of Origin | (C57BL/6J x DBA/2NCrl)F2 |
Chromosome | UN |
Molecular Note | The alpha-MHC-mCherry transgene contains a cardiac-specific alpha myosin heavy-chain (Myh6) mousepromoter sequence upstream of an mCherry fluorescent protein cDNA sequence. The modified mouse Myh6 promoter sequence is minimally active/attenuated through the ablation of three GATA sites and twothyroid-like response elements, thereby directing cardiac-specific mCherry expression and decreased background expression. Founder line 2 were found to have extremely bright mCherry fluorescence in the heart at all stages of development. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype (noncarrier) mice from the colony, or to C57BL/6J inbred mice. The donating investigator reports that homozygous mice are viable and fertile, with no reported phenotypic abnormalities.
When using the αMHC-mCherry mouse strain in a publication, please cite the originating article(s) and include JAX stock #021577 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Myh6*-mCherry)2Mik |
Frozen Mouse Embryo | B6;D2-Tg(Myh6*-mCherry)2Mik/J | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(Myh6*-mCherry)2Mik/J | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(Myh6*-mCherry)2Mik/J | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(Myh6*-mCherry)2Mik/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.