FCGRT-deficient BXSB.Yaa mice (BXSB.Yaa FCGRT-/- or BXSB.Yaa FcRn-/-) are a BXSB-congenic strain carrying an FcRn null mutation. These mice may be useful in studying the role of the FcRn pathway in spontaneous lupus-like autoimmune syndrome.
Dr. Derry Roopenian, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Yaa | accelerated autoimmunity and lymphoproliferation transposition |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fcgrt | Fc receptor, IgG, alpha chain transporter |
FCGRT-deficient BXSB.Yaa mice (BXSB.Yaa FCGRT-/- or BXSB.Yaa FcRn-/-) are a BXSB-congenic strain carrying an FcRn null mutation. Homozygous (FCGRT-/-) mice lack the Fc receptor (FcRn), which normally functions to enhance the presentation of immune-complexed antigens by MHC class II molecules and is critical for maintaining normal concentrations of immunoglobulin G (IgG) antibodies and serum albumin.
Homozygous mice are viable and fertile. Both homozygous and heterozygous mice develop spontaneous lupus-like autoimmune syndrome similarly to BXSB/MpJ inbred mice (Stock No. 000740): mortality in males starts at ~13 weeks of age with 50% lethality by ~30 weeks and 76% lethality by ~40 weeks. Females develop a greatly attenuated form of autoimmune disease because they lack Yaa. The donating investigator also reports that FcRn-/- mice exhibit ~50% reduction in IgG.
C57BL/6J-congenic mice harboring the FcRn null allele are described and available from The Jackson Laboratory Repository as Stock No. 003982.
The FcRn null allele (Fcgrttm1Dcr) was designed by Dr. Derry C. Roopenian (The Jackson Laboratory) with a PGK-neomycin cassette replacing exons 1-2 of the Fcgrt gene (Fc receptor, IgG, alpha chain transporter) on chromosome 7. C57BL/6J-congenic mice harboring this FcRn null allele are described and available from The Jackson Laboratory Repository as Stock No. 003982.
Dr. Roopenian backcrossed mice harboring the FcRn null mutation with BXSB/MpJ inbred mice (Stock No. 000740) for 11 generations, and then maintained the colony by breeding homozygous mice together. In 2012, Dr. Roopenian froze sperm from BXSB.Yaa FCGRT-/- mice at generation N11F30. In 2013, this frozen sperm was transferred to The Jackson Laboratory Repository (Autoimmune Resource) to establish Stock No. 021455. Male mice have the BXSB/MpJ-derived Y chromosome that contains the Y-linked autoimmune accelerator locus (Yaa).
Allele Name | accelerated autoimmunity and lymphoproliferation |
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Allele Type | Spontaneous |
Allele Synonym(s) | Is(XOfd1-Mid1;Y)1Mp; Tp(X;Y)1Ekw |
Gene Symbol and Name | Yaa, accelerated autoimmunity and lymphoproliferation transposition |
Gene Synonym(s) | |
Strain of Origin | SB/Le |
Chromosome | Y |
Molecular Note | An approximately 4 MB region of the X chromosome that includes at least 13 known genes (spanning from Ofd1 to Mid1) was translocated to the Y chromosome adjacent to the pseudoautosomal region. Increased RNA expression of Msl3, Tlr7, Tmsb4x and Rab9 was detected in follicular B cells. |
Allele Name | targeted mutation 1, Derry C Roopenian |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | FcRn- |
Gene Symbol and Name | Fcgrt, Fc receptor, IgG, alpha chain transporter |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 7 |
Molecular Note | Sequence from exon 1 and part of exon2 was replaced with a PGK-neo cassette. Quantitative PCR of liver cDNA indicated the absence of mRNA. Western blot analysis of neonatal intestinal extracts failed to reveal protein product. |
To maintain the live colony, homozygous mice may be bred together. Both homozygous and heterozygous mice develop spontaneous lupus-like autoimmune syndrome similarly to BXSB/MpJ inbred mice (Stock No. 000740): mortality in males starts at ~13 weeks of age with 50% lethality by ~30 weeks and 76% lethality by ~40 weeks. Females develop a greatly attenuated form of autoimmune disease because they lack Yaa. The expected coat color is white-bellied agouti.
When using the FcRn- mouse strain in a publication, please cite the originating article(s) and include JAX stock #021455 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Fcgrt<tm1Dcr> |
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