These mice possess loxP sites flanking exons 2 through 5 of the ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) (Elavl1) gene and have applications in studies related to mRNA stability and regulation of gene expression.
Timothy Hla, Weill Cornell Medical College
Elavl1 encodes an RNA-binding protein that binds to AU-rich elements of the 3' UTR of mRNAs. These mice possess loxP sites on either side of exons 2 through 5 of the Elavl1 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of regulation of post-transcriptional gene expression in angiogenesis.
A targeting vector designed by Dr. Timothy Hla (Weill Cornell Medical College) was used to insert a loxP site upstream of exon 2, and a FRT site and loxP site downstream of exon 5. This construct was electroporated into unspecified 129/Sv derived embryonic stem (ES) cells. Correctly targeted ES cells (exons 2 through 5, including the FRT site, flanked by loxP sites) were injected into blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6 mice to establish the colony. The donating investigator reported the mice were backcrossed to C57BL/6 mice for a total of five generations (see SNP results below) prior to sending males to The Jackson Laboratory Repository in 2012. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
In 2013, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating (3 markers were heterozygous in all mice; 1 marker heterozygous in a few mice). These data show that the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6N;C57BL/6J genetic background.
|Allele Name||targeted mutation 1, Timothy Hla|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Elavl1, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R)|
|Strain of Origin||129|
|Molecular Note||A loxP site was inserted upstream of exon 2 and an frt and loxP site was inserted downstream of exon 5.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Elavl1f mouse strain in a publication, please cite the originating article(s) and include JAX stock #021431 in your Materials and Methods section.