In this strain a NEO cassette replaces exon 1 of the Foxo6 (forkhead box O6) gene. These mice exhibit impaired memory consolidation and abnormal hippocampal neuron morphology.
Anne Brunet, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Foxo6 | forkhead box O6 |
The transcription factor forkhead box O6 is expressed primarily in the central nervous system (predominantly in the hippocampal CA1 and CA3 regions), and is negatively regulated by the insulin and insulin-like growth factor (IGF) signaling pathway. These mice carry a targeted mutation of the mouse Foxo6, forkhead box O6, gene in which a NEO cassette replaced exon 1. Mice that are homozygous for this targeted mutation are viable and fertile, but have smaller litter sizes compared to wildtype controls. No full-length gene product (mRNA or protein) is detected by Western blot analysis of the anterior brain, cortex, hippocampus and testes or by RT-PCR analysis of hippocampus. Homozygotes exhibit impaired memory consolidation, as indicated by contextual memory tests. Reduced dendritic spine density of hippocampal neurons is observed in cultured hippocampal neurons and dendritic spine length is also increased in the CA1 region of homozygous mice. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector designed by Dr. Anne Brunet (Stanford University School of Medicine) containing a NEO cassette was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then backcrossed to C57BL/6J for 11 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Anne Brunet |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Foxo6, forkhead box O6 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 4 |
Molecular Note | A neo cassette replaced exon 1. Western blot analysis confirmed the absence of protein expression in the anterior brain, cortex and hippocampus. |
When maintaining a live colony, these mice can be bred as homozygotes. Homozygotes are viable and fertile, but have smaller litter sizes.
When using the B6.129-Foxo6tm1Abru/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021347 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Foxo6<tm1Abru> |
Frozen Mouse Embryo | B6.129-Foxo6<tm1Abru>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Foxo6<tm1Abru>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Foxo6<tm1Abru>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Foxo6<tm1Abru>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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