In this strain, a neo cassette replaces entire coding region of the Mediterranean fever (Mefv) gene, abolishing expression. Mefv encodes pyrin, a protein expressed by cells of myeloid lineage. Pyrin is involved in the formation of the inflammasome which regulates the immune system's response to injury, toxins, and infection. Mutations in Mefv have been associated with Familial Mediterranean Fever (FMF), an autoinflammatory disorder characterized by unprovoked episodes of fever and inflammation. Homozygous Mefv-deficient mice are viable and fertile. These mice exhibit enhanced interleukin (IL)-1β release by macrophages in response to inflammatory stimuli. Lipopolysaccharide (LPS) treatment results in hypothermia, and a decrease in body weight accompanied by local neutrophilic inflammation.
A targeting vector was designed to replace entire coding region of the Mediterranean fever (Mefv) gene with a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129S6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to 129S6/SvEvTac females. Upon arrival at The Jackson Laboratory, mice were bred to 129S6 inbred mice for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Beverly H Koller|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mefv, Mediterranean fever|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A targeting vector was designed to replace entire coding region with a neomycin resistance (neo) cassette in reverse orientation to the gene. Northern blot analysis confirmed the absence of transcript expression in elicited peritoneal neutrophils.|
When maintaining a live colony, homozygous mice may be bred together.
When using the 129S6/SvEvTac-Mefvtm1Bhk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021315 in your Materials and Methods section.