| Allele Name | transgene insertion 3, Joshua R Sanes |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Brainbow 3.1 line 3; TSOGK3 |
| Gene Symbol and Name | Tg(Thy1-Brainbow3.1)3Jrs, transgene insertion 3, Joshua R Sanes |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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| Expressed Gene | RFP, Red Fluorescent Protein, coral |
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| Expressed Gene | OFP, Orange Fluorescent Protein, jelly fish |
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| Site of Expression | Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells. |
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| Strain of Origin | C57BL/6J x CBA |
| Chromosome | UN |
| Molecular Note | The Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (phiNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. Line 3 was generated. |
