These Brainbow 3.1 (founder line 3) mice allow labeling of individual neuronal types (including motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells) with up to 90 distinguishable color variations in cre recombined cells.
Joshua R Sanes, Harvard University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These Thy1-Brainbow 3.1 (line 3) transgenic mice are viable and fertile as hemizygotes. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible lox recognition sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, no fluorescent protein is expressed. When bred to Cre recombinase expressing mice, the resulting offspring can have one of four expression outcomes for each transgene in each cell of the cre expressing tissue(s): a non-fluorescent nuclear-localized protein that can be immunostained (no recombination), eGFP, mOrange2 or mKate2. Farnesylation sequences tethers fluorescent proteins to the membrane, allowing clear labeling of fine processes. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells. Other areas have not been tested. The Donating Investigator has not attempted to make the strain homozygous to date (March 2013).
The Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (ΦNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. Founder line 3 mice were bred with C57BL/6 and CD1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | OFP, Orange Fluorescent Protein, jelly fish |
Site of Expression | Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells. |
Allele Name | transgene insertion 3, Joshua R Sanes |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Brainbow 3.1 line 3; TSOGK3 |
Gene Symbol and Name | Tg(Thy1-Brainbow3.1)3Jrs, transgene insertion 3, Joshua R Sanes |
Gene Synonym(s) | |
Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Expressed Gene | OFP, Orange Fluorescent Protein, jelly fish |
Site of Expression | Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells. |
Strain of Origin | C57BL/6J x CBA |
Chromosome | UN |
Molecular Note | The Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (phiNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. Line 3 was generated. |
When maintaining a live colony, hemizygous mice are bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous to date (March 2013).
When using the STOCK Tg(Thy1-Brainbow3.1)3Jrs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021225 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Thy1-Brainbow3.1)3Jrs |
Frozen Mouse Embryo | STOCK Tg(Thy1-Brainbow3.1)3Jrs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Thy1-Brainbow3.1)3Jrs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Thy1-Brainbow3.1)3Jrs/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Thy1-Brainbow3.1)3Jrs/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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