These Thy1-Brainbow 3.1 (line 3) transgenic mice are viable and fertile as hemizygotes. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible <i>lox</i> recognition sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, no fluorescent protein is expressed. When bred to Cre recombinase expressing mice, the resulting offspring can have one of four expression outcomes for each transgene in each cell of the cre expressing tissue(s): a non-fluorescent nuclear-localized protein that can be immunostained (no recombination), eGFP, mOrange2 or mKate2. Farnesylation sequences tethers fluorescent proteins to the membrane, allowing clear labeling of fine processes. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells.  Other areas have not been tested.  The Donating Investigator has not attempted to make the strain homozygous to date (March 2013).

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These Brainbow 3.1 (founder line 3) mice allow labeling of individual neuronal types (including motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells) with up to 90 distinguishable color variations in <i>cre</i> recombined cells.

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STOCK Tg(Thy1-Brainbow3.1)3Jrs/J Frozen Embryo