This strain carries a floxed allele of Trp53cor1 (tumor protein p53 pathway corepressor 1; also known as LincRNA-p21) and is useful in studies of cell cycle regulation, apoptosis and cancer.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Trp53cor1 (tumor protein p53 pathway corepressor 1; also known as LincRNA-p21) is a Trp53-regulated long intergenic non-coding RNA (lincRNA) expressed in the Cdkn1a (p21) locus on mouse Chromosome 17.
Floxed mutant animals were generated by flanking the promoter region and exon 1 of LincRNA-p21 with loxP sites. In the floxed configuration, expression is not affected. Upon Cre excision of the floxed region, expression is prevented. Homozygous floxed mice are viable and fertile and do not exhibit any overt phenotypes. This strain is useful in studies of cell cycle regulation, apoptosis and cancer.
The promoter region and first exon were flanked by loxP sites and an FRT-Neo-FRT drug selection cassette was placed downstream of exon 1, just inside the floxed region. The mutation was created in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. The neomycin resistance cassette was subsequently removed by breeding founders to a C57BL/6 background beta-actin FlpO strain. This strain was maintained on a mixed C57BL/6-129S4 genetic background by the donating laboratory.
|Allele Name||targeted mutation 1.1, Tyler Jacks|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Trp53cor1, tumor protein p53 pathway corepressor 1|
|Gene Synonym(s)||Gm16197; Gm16197; OTTMUSG00000031656; TRP53COR1; linc-p21; lincRNA-p21; predicted gene 16197; predicted gene, OTTMUSG00000031656|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||The promoter region and first exon, which includes the Trp53 response element (p53RE), are flanked by loxP sites and an FRT-Neo-FRT drug selection cassette is placed downstream of exon 1, just inside the floxed region. Flp-mediated recombination removed the neo cassette.|
Homozygous floxed mice are viable and fertile.
When using the STOCK Trp53cor1tm1.1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021214 in your Materials and Methods section.
|Heterozygous for Trp53cor1<tm1.1Tyj>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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