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B6(129S4)-Mirc32tm1.1Tyj/J
Stock No: 021213
  • Targeted Mutation
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  • Overview
  • Details
    • Detailed Description
    • Development
    • Control Suggestions
    • Selected References
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
  • Technical Support
    • Genotyping Protocols
    • Breeding Considerations
    • Citation
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  • Related Strains

Overview

Mir143 (microRNA 143) and Mir145 (microRNA 145) are flanked by loxP sites in this targeted mutation strain, enabling the generation of Cre-mediated, tissue-specific knockouts that affect smooth muscle function.

Donating Investigator

Dr. Tyler Jacks, Massachusetts Institute of Technology

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Genetic overview

Genetic Background Generation

Mirc32tm1.1Tyj

Allele Type Gene Symbol Gene Name
Targeted (Null/Knockout) Mirc32 microRNA cluster 32, including Mir143 and Mir145a
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Research Applications

  • Research Tools
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Details

Detailed Description

The mir-143~145 cluster on mouse Chromosome 18 encodes two unrelated miRNAs, Mir143 (microRNA 143) and Mir145 (microRNA 145). Germline deletion of the cluster leads to a range of smooth muscle-related phenotypes (including megacolon, vascular defects and male infertility) that occur with high penetrance and progress with aging.


Mir143 and Mir145 are flanked by loxP sites in this targeted mutation strain, thus enabling Cre-mediated recombination and excision of the cluster. In the floxed configuration, miRNA expression is not affected. Homozygotes are viable, fertile, and do not exhibit any overt phenotypes.

Development

LoxP sites were introduced approximately 250 bp 5' of Mir145 and 500 bp 3' of Mir143. A FRT-Neo-FRT cassette was also placed 3' of Mir143, just inside the floxed region. The mutation was created in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. The neomycin cassette was removed by breeding founders to a C57BL/6 background beta-actin FlpO strain. This strain was made congenic with C57BL/6 using speed congenic methods by the donating laboratory.

Control Suggestions

  • 000664 C57BL/6J

Additional Information

  • Considerations for Choosing Controls

Selected References

  • Dimitrova N; Gocheva V; Bhutkar A; Resnick R; Jong RM; Miller KM; Bendor J; Jacks T. 2016. Stromal Expression of miR-143/145 Promotes Neoangiogenesis in Lung Cancer Development. Cancer Discov 6(2):188-201PubMed: 26586766MGI: J:229235
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Genetics

Mirc32tm1.1Tyj

Allele Symbol: Mirc32tm1.1Tyj

Allele Name targeted mutation 1.1, Tyler Jacks
Allele Type Targeted (Null/Knockout)
Allele Synonym(s) miR-143/145F; Mir145/Mir143tm1.1Tyj
Gene Symbol and Name Mirc32, microRNA cluster 32, including Mir143 and Mir145a
Gene Synonym(s)
Strain of Origin (C57BL/6 x 129S4/SvJae)F1
Chromosome 18
Molecular Note LoxP sites were introduced approximately 250 bp 5' of Mir145 and 500 bp 3' of Mir143. A FRT-Neo-FRT cassette was also placed 3' of Mir143, just inside the floxed region. Flp-mediated recombination removed the neo cassette.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

  • Research Tools
    • Internal/Organ Research
    • Reproductive Biology Research
      • Cre-lox System
    • Cre-lox System
      • loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

References

  • Dimitrova N; Gocheva V; Bhutkar A; Resnick R; Jong RM; Miller KM; Bendor J; Jacks T. 2016. Stromal Expression of miR-143/145 Promotes Neoangiogenesis in Lung Cancer Development. Cancer Discov 6(2):188-201PubMed: 26586766MGI: J:229235

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • Standard PCR:Mirc32
    • Genotyping resources and troubleshooting
  • Breeding Considerations

    Homozygous and heterozygous floxed mice are viable and fertile.

    • Additional Breeding and Husbandry Support
  • Citation

    When using the B6(129S4)-Mirc32tm1.1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021213 in your Materials and Methods section.

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