This floxed-Bin1 strain may be useful for studying the cellular and mechanical role of Bin1 in Alzheimer's disease and the attenuation of cancer.
George Prendergast, Lankenau Institute for Medical Research
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Bin1 | bridging integrator 1 |
These Bin1flox mice possess loxP sites flanking exon 3 of the bridging integrator 1 (Bin1) gene. BIN1 is a nucleocytoplasmic adaptor protein, involved in phagocytosis, apoptosis, and synaptic vesicle endocytosis. BIN1 has been implicated in the development of cardiac defects and Alzheimer's disease and has been shown to attenuate many types of cancer. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues. Widespread deletion results in perinatal lethality due to ventricular cardiomyopathy.
When crossed to B6129-Tg(Wap-cre)11738Mam/J mice (Stock No. 003552) expressing Cre recombinase in mammary gland tissues, no long term increase in breast cancer incidence was observed in either virgin or parous animals, however, initiation with the Ras-activating carcinogen DMBA (7,12-dimethylbenz(a)anthracene) produced higher grade, more
poorly differentiated mammary gland tumors.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of exon 3, and a single mutant loxP site, with a T to C point mutation, downstream of exon 3 of the bridging integrator 1 (Bin1) gene. The construct was electroporated into 129S6/SvEvTac-derived IT2 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exon 3, intact floxed-neo cassette, or excision of both exon 3 and the neo cassette. The mutant loxP site allowed cre-recombination to favor removal of the neo cassette rather than exon 3. Correctly targeted ES cells, containing only the floxed-exon 3, were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting offspring were bred to establish a colony of Bin1flox mice. These mice were backcrossed to C57BL/6J for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 2, George C Pendergast |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Bin1flox |
Gene Symbol and Name | Bin1, bridging integrator 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 18 |
Molecular Note | LoxP sites flank exon 3. The 3' loxP site retained in the floxed ES cell line used to generate chimeric mouse includes a point mutant which reduces the efficiency of in vivo recombination ~50%. |
When maintaining a live colony, homozygotes may be bred together.
When using the B6.129S6-Bin1tm2Gcp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021145 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bin1<tm2Gcp> |
Frozen Mouse Embryo | B6.129S6-Bin1<tm2Gcp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bin1<tm2Gcp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bin1<tm2Gcp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Bin1<tm2Gcp>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.