B6N.129-Neto2^tm1Mci/J

Stock number: 021062

Description

These Neto2, neuropilin (NRP) and tolloid (TLL)-like 2, targeted mutation mice exhibit impaired neuronal potassium chloride (KCC2) co-transporter function and have applications in studies of ionotropic glutamate receptors and synaptic plasticity.

Detailed description

Neuropilin (NRP) and tolloid (TLL)-like 2 contains two extracellular CUB domains and a low-density lipoprotein class A (LDLa) domain and functions as an auxillary subunit of neuronal kainate-sensitive glutamate receptors. These mice carry a targeted mutation of the Neto2 (Neuropilin (NRP) and tolloid (TLL)-like 2 contains 2) gene, in which exons 3 through 6 are replaced by a NEO cassette. Homozygotes are viable and fertile. No gene product (protein) is detected by immunoblot analysis of brain lysates from homozygotes. Mutant mice exhibit impaired neuronal potassium chloride (KCC2) co-transporter activity and reduced KCC2 protein levels. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6N genetic background.

Development

Dr. Roderick R. McInnes, while at University of Toronto, designed a targeting vector that contains a NEO cassette to disrupt exons 3 through 6 of the targeted gene. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6NCrl for more than 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6NCrl genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Neto2^tm1Mci
Name: targeted mutation 1, Roderick R McInnes
MGI accession ID: MGI:5141222
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Neto2
Marker name: neuropilin (NRP) and tolloid (TLL)-like 2
Chromosome: 8
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exons 3 - 6 were replaced with a neo cassette via homologous recombination. Immunoblot analysis of brain lysates from homozygous mice confirms the absence of protein expression.