These Neto2, neuropilin (NRP) and tolloid (TLL)-like 2, targeted mutation mice exhibit impaired neuronal potassium chloride (KCC2) co-transporter function and have applications in studies of ionotropic glutamate receptors and synaptic plasticity.
Roderick R. McInnes, Hospital for Sick Children Research Inst
Neuropilin (NRP) and tolloid (TLL)-like 2 contains two extracellular CUB domains and a low-density lipoprotein class A (LDLa) domain and functions as an auxillary subunit of neuronal kainate-sensitive glutamate receptors. These mice carry a targeted mutation of the Neto2 (Neuropilin (NRP) and tolloid (TLL)-like 2 contains 2) gene, in which exons 3 through 6 are replaced by a NEO cassette.
Homozygotes are viable and fertile. No gene product (protein) is detected by immunoblot analysis of brain lysates from homozygotes. Mutant mice exhibit impaired neuronal potassium chloride (KCC2) co-transporter activity and reduced KCC2 protein levels.
During backcrossing, the Y chromosome may not have been fixed to the C57BL/6N genetic background.
Dr. Roderick R. McInnes, while at University of Toronto, designed a targeting vector that contains a
NEO cassette to disrupt exons 3 through 6 of the targeted gene. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6NCrl for more than 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6NCrl genetic background.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ
(Stock No. 005304) at least once to establish the colony.
|Allele Name||targeted mutation 1, Roderick R McInnes|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Neto2, neuropilin (NRP) and tolloid (TLL)-like 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exons 3 - 6 were replaced with a neo cassette via homologous recombination. Immunoblot analysis of brain lysates from homozygous mice confirms the absence of protein expression.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6N.129-Neto2tm1Mci/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021062 in your Materials and Methods section.