These Neto1, neuropilin (NRP) and tolloid (TLL)-like 1, targeted mutation mice exhibit reduced long-term potentiation and deficits in hippocampal-dependent spatial learning. This strain has applications in studies of the N-methyl-D-aspartate (NMDA) receptor complex and synaptic plasticity.
Roderick R. McInnes, Hospital for Sick Children Research Inst
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Neto1 | neuropilin (NRP) and tolloid (TLL)-like 1 |
The synaptic membrane protein, neuropilin (NRP) and tolloid (TLL)-like 1, has 2 extracellular CUB domains and a low-density lipoprotein class A (LDLa) domain, and is a component of the synaptic N-methyl-D-aspartate (NMDA) receptor complex. These mice carry a targeted mutation of the Neto1 (Neuropilin (NRP) and tolloid (TLL)-like 1) gene, in which exons 3 through 6 are replaced by a tau-lacZ and floxed NEO cassette. Homozygotes are viable and fertile. No gene product (protein) is detected by immunoblot analysis of brain lysates from homozygotes. Mutant mice that are homozygous for the targeted mutation have fewer NMDA receptors and reduced synaptic kainate receptor currents (kainate receptor mediated excitatory postsynaptic potential) when compared to wildtype mice. Homozygotes exhibit impaired NMDAR (N-methyl-D-aspartate receptor) - dependent spatial learning and memory. The Donating Investigator reports that the beta?galactosidase, lacZ reporter, activity is detected in only a subset of neurons that express Neto1 mRNA. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
Dr. Roderick R. McInnes, while at University of Toronto, designed a targeting vector, containing a floxed NEO cassette and a tau-lacZ (tlz) cassette, to disrupt exons 2 and 3 of the targeted gene. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J mice. The mice were then backcrossed to C57BL/6J for 2 generations and then backcrossed to C57BL/6NCrl for more than 10 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ
(Stock No. 005304) at least once to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Roderick R McInnes |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Neto1tlz |
Gene Symbol and Name | Neto1, neuropilin (NRP) and tolloid (TLL)-like 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 18 |
Molecular Note | Exon 2 and 3 were replaced with a tau-lacZ (tlz) and floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6N.129-Neto1tm1Mci/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #021061 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Neto1<tm1Mci> |
Frozen Mouse Embryo | B6N.129-Neto1<tm1Mci>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129-Neto1<tm1Mci>/J | $2595.00 |
Frozen Mouse Embryo | B6N.129-Neto1<tm1Mci>/J | $3373.50 |
Frozen Mouse Embryo | B6N.129-Neto1<tm1Mci>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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