129S-Jdp2^tm1Kmm/J

Stock number: 021056
Product name: Jdp2 KO
Research areas: Apoptosis Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These Jun dimerization protein 2 (Jdp2) deficient mice may be useful when studying osteoclastogenesis and neutrophil mediated immune defenses.

Detailed description

A loxP-flanked neo cassette replaces exon 2 of the Jun dimerization protein 2 (Jdp2) gene, abolishing gene expression. JDP2 is a component of the AP-1 transcription factor that represses Jun-mediated roles such as apoptosis, osteoclastogenesis, and neutrophil homeostasis. Mice that are homozygous for this allele are viable and fertile. These Jdp2^-/- mice exhibit reduced expression of lymphocyte antigen 6G (Ly6G) compared to wild-type mice.

Development

A targeting vector was designed to replace the exon 2, containing the Start-ATG, of the AP-1 family transcription factor Jun dimerization protein 2 (Jdp2) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ-22 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6-derived blastocysts and resulting chimeric males were bred with 129S6/SvEvTac inbred females to establish a colony of Jdp2-/- mice. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for for at least one generation to establish the colony.

Allele

Symbol: Jdp2^tm1Kmm
Name: targeted mutation 1, Kenneth Murphy
MGI accession ID: MGI:5445757
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Jdp2
Marker name: Jun dimerization protein 2
Marker synonyms: JUNDM2; Jundp2; TIF
Chromosome: 12
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to replace the exon 2, containing the Start-ATG with a loxP-flanked neomycin resistance (neo) cassette.
General note: ES cell = EDJ-22 (129S6/SvEvTac)