These Jun dimerization protein 2 (Jdp2) deficient mice may be useful when studying osteoclastogenesis and neutrophil mediated immune defenses.
Dr. Kenneth Murphy, Washington University School of Medicine
A loxP-flanked neo cassette replaces exon 2 of the Jun dimerization protein 2 (Jdp2) gene, abolishing gene expression. JDP2 is a component of the AP-1 transcription factor that represses Jun-mediated roles such as apoptosis, osteoclastogenesis, and neutrophil homeostasis. Mice that are homozygous for this allele are viable and fertile. These Jdp2-/- mice exhibit reduced expression of lymphocyte antigen 6G (Ly6G) compared to wild-type mice.
A targeting vector was designed to replace the exon 2, containing the Start-ATG, of the AP-1 family transcription factor Jun dimerization protein 2 (Jdp2) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ-22 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6-derived blastocysts and resulting chimeric males were bred with 129S6/SvEvTac inbred females to establish a colony of Jdp2-/- mice. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Kenneth Murphy|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Jdp2, Jun dimerization protein 2|
|Strain of Origin||129S6/SvEvTac|
|General Note||ES cell = EDJ-22 (129S6/SvEvTac)|
|Molecular Note||A targeting vector was designed to replace the exon 2, containing the Start-ATG with a loxP-flanked neomycin resistance (neo) cassette.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Jdp2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #021056 in your Materials and Methods section.