Overview

Cx3cr1^CreER knock-in/knock-out mice express tamoxifen-inducible Cre recombinase under the direction of the Cx3cr1 promoter in the mononuclear phagocyte system making them useful for fate-mapping studies of the monocyte and macrophage compartment, as well as microglia.

Donating Investigator

Steffen Jung, Weizmann Institute of Science

Genetic overview

Genetic Background	Generation
	N10+pN3 (2019-03-01 00:00:00)

Cx3cr1^{tm2.1(cre/ERT2)Jung}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Cx3cr1	chemokine (C-X3-C motif) receptor 1

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

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Details

Detailed Description

Cx3cr1^CreER knock-in/knock-out mice express a Cre-ER fusion protein from endogenous Cx3cr1 promoter/enhancer elements. Insertion of the Cre-ER fusion protein knocks out endogenous CX3CR1 expression. CX3CR1 is a chemokine receptor expressed in the mononuclear phagocyte system, as well as microglia. Homozygous mice are viable and fertile. When Cx3cr1^CreER mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences.

For example, when bred to B6.129X1-Gt(ROSA)26Sor^tm1(EYFP)Cos/J mice (Stock No. 006148), cre-mediated recombination results in yellow fluorescent protein expression in microglia, and intestinal and kidney macrophages.

Additionally, very low levels of Cre activity may be present prior to introduction of tamoxifen - but the Cre activity after tamoxifen are significantly greater than those baseline levels. As such, it is recommended that researchers include tamoxifen-negative controls to establish any baseline levels of Cre activity in their experiments. For example, when Cx3cr1^CreER are bred to the dual fluorescent reporter line mT/mG (Stock No. 007676), the resulting mice had some basal Cre activity (GFP expression) in a fraction of microglia prior to tamoxifen treatment, but the Cre activity upon tamoxifen treatment was significantly greater (almost completely restricted to microglia).

Of note, The Jackson Laboratory also has available Cx3cr1^CreER mice with EYFP expression (Stock No. 021160).

Development

A targeting vector was designed to replace exon 1 of the chemokine (C-X3-C) receptor 1 (Cx3cr1) gene with a CreER (Cre recombinase fused to an estrogen receptor ligand binding domain) coding sequence and a loxP-flanked neomycin (neo) resistance cassette. This construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Chimeras were made by ES cell aggregation with eight-cell stage embryos and chimeric mice were bred with Tg(Pgk1-cre)1Lni mice to delete the neo cassette. Resulting offspring were bred to remove the cre-expressing transgene, and progeny were bred with C57BL/6JOlaHsd inbred mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Mice express tamoxifen-inducible Cre recombinase in the mononuclear phagocyte system.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Yona S; Kim KW; Wolf Y; Mildner A; Varol D; Breker M; Strauss-Ayali D; Viukov S; Guilliams M; Misharin A; Hume DA; Perlman H; Malissen B; Zelzer E; Jung S. 2013. Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. Immunity 38(1):79-91PubMed: 23273845 MGI: J:193038

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Genetics

Cx3cr1^{tm2.1(cre/ERT2)Jung}

Allele Symbol: Cx3cr1^{tm2.1(cre/ERT2)Jung}

Allele Name	targeted mutation 2.1, Steffen Jung
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Cx3cr1^{tm2.1(cre/ERT2)Jung}; targeted mutation 2.1, Steffen Jung
Gene Symbol and Name	Cx3cr1, chemokine (C-X3-C motif) receptor 1
Gene Synonym(s)	CMKDR1; GPR13; GPRV28; V28; CMKBRL1; CCRL1; Rbs11
Promoter	Cx3cr1, chemokine (C-X3-C motif) receptor 1, mouse, laboratory
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Mice express tamoxifen-inducible Cre recombinase in the mononuclear phagocyte system.
Strain of Origin	129P2/OlaHsd
Chromosome	9
Molecular Note	A cre/ERT2 cassette with a floxed neo gene was inserted by RedE/T recombineering into a BAC containing the Cx3cr1 locus, replacing the coding exon of Cx3cr1. Properly targeted ES cells were aggregated with 8-cell embryos to generate chimeras. Chimeras were backcrossed to C57BL/6 mice. Tne neo was excised by crossing the animals to a cre deleter strain (Tg(Pgk1-cre)1Lni).

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Yona S; Kim KW; Wolf Y; Mildner A; Varol D; Breker M; Strauss-Ayali D; Viukov S; Guilliams M; Misharin A; Hume DA; Perlman H; Malissen B; Zelzer E; Jung S. 2013. Fate Mapping Reveals Origins and Dynamics of Monocytes and Tissue Macrophages under Homeostasis. Immunity 38(1):79-91PubMed: 23273845 MGI: J:193038

Additional References

Gentek R; Ghigo C; Hoeffel G; Jorquera A; Msallam R; Wienert S; Klauschen F; Ginhoux F; Bajenoff M. 2018. Epidermal gammadelta T cells originate from yolk sac hematopoiesis and clonally self-renew in the adult. J Exp Med 215(12):2994-3005PubMed: 30409784 MGI: J:269027
Kreisel T; Wolf B; Keshet E; Licht T. 2019. Unique role for dentate gyrus microglia in neuroblast survival and in VEGF-induced activation. Glia 67(4):594-618PubMed: 30453385 MGI: J:271655
Rotterman TM; Akhter ET; Lane AR; MacPherson KP; Garcia VV; Tansey MG; Alvarez FJ. 2019. Spinal Motor Circuit Synaptic Plasticity after Peripheral Nerve Injury Depends on Microglia Activation and a CCR2 Mechanism. J Neurosci 39(18):3412-3433PubMed: 30833511 MGI: J:274670
Culemann S; Gruneboom A; Nicolas-Avila JA; Weidner D; Lammle KF; Rothe T; Quintana JA; Kirchner P; Krljanac B; Eberhardt M; Ferrazzi F; Kretzschmar E; Schicht M; Fischer K; Gelse K; Faas M; Pfeifle R; Ackermann JA; Pachowsky M; Renner N; Simon D; Haseloff RF; Ekici AB; Bauerle T; Blasig IE; Vera J; Voehringer D; Kleyer A; Paulsen F; Schett G; Hidalgo A; Kronke G. 2019. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature 572(7771):670-675PubMed: 31391580 MGI: J:283709

Additional - Cx3cr1^{tm2.1(cre/ERT2)Jung} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Cx3cr1^{tm2.1(cre/ERT2)Jung}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the B6.129P2(C)-Cx3cr1^{tm2.1(cre/ERT2)Jung}/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #020940 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available Now

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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for x3cr1<tm2.1(cre/ERT2)Jung>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Cx3cr1tm2.1(cre/ERT2)Jung

Allele Symbol: Cx3cr1tm2.1(cre/ERT2)Jung

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Cx3cr1tm2.1(cre/ERT2)Jung related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cx3cr1^{tm2.1(cre/ERT2)Jung}

Allele Symbol: Cx3cr1^{tm2.1(cre/ERT2)Jung}

Additional - Cx3cr1^{tm2.1(cre/ERT2)Jung} related