B6.Cg-Tg(tetO-Mif)279Aren/J

Stock number: 020652
Research areas: Research Tools

Description

These transgenic mice express mouse Mif (macrophage migration inhibitory factor)under the control of a tetracycline operator (tetO). When combined with a rat Clara-cell secretory protein (CCSP) promoter/rtTA allele, the lung-directed overexpression of MIF is sufficient to promote Lewis lung carcinoma (LLC)-initiated tumor growth after two weeks of doxycycline treatment.

Detailed description

MIF (macrophage migration inhibitory factor, murine) is a cytokine that is expressed during tissue repair and tumor growth and has been shown to inhibit apoptosis. It is expressed in the lung in response to induced bleomycin and naphthalene damage of the epithelium.
These transgenic mice express mouse Mif under the control of a tetracycline operator (tetO). When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), MIF expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring.
When combined with a rat Clara-cell secretory protein (CCSP) promoter/rtTA allele (see Stock No. 006232), the lung-directed overexpression of MIF is sufficient to promote Lewis lung carcinoma (LLC)-initiated tumor growth after two weeks of doxycycline treatment.
The coat color of hemizygous and wildtype mice may be either black or agouti.

Development

Mouse Mif cDNA isolated from C57BL/6-derived Lewis lung carcinoma (LLC) cells was inserted into an expression cassette consisting of seven tetO repeats, a cytomegalovirus minimal promoter, and a bovine growth hormone polyadenylation sequence. The transgenic vector was introduced to B6SJL hybrid embryos. The donating investigator reported the mice were backcrossed to C57BL/6 mice for at least seven generations (see SNP results below) prior to sending males to The Jackson Laboratory Repository in 2013. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

In 2013, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. While 26 of 27 markers were fixed for C57BL/6 allele-type, one marker on chromosome 1 was not C57BL/6 allele-type in some mice tested (e.g., still segregating for SJL allele-type). In addition, 4 of 5 markers that determine C57BL/6J from C57BL/6N were also found to be segregating on chromosomes 8, 13, 15 and 19 (note that these markers are the same for C57BL/6N and SJL). These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N or a mixed C57BL/6N;C57BL/6J genetic background.

Allele

Symbol: Tg(tetO-Mif)279Aren
Name: transgene insertion 279, Douglas Arenberg
MGI accession ID: MGI:5461057
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-Mif)279Aren
Marker name: transgene insertion 279, Douglas Arenberg
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: Mif,macrophage migration inhibitory factor (glycosylation-inhibiting factor),mouse, laboratory
Molecular note: Mouse Mif cDNA isolated from C57BL/6-derived Lewis lung carcinoma (LLC) cells was inserted into an expression cassette consisting of seven tetO repeats, a cytomegalovirus minimal promoter, and a bovine growth hormone polyadenylation sequence. Line 279 was generated.