B6;129S6-Mir193b^tm1.1Mam/J

Stock number: 020100
Research areas: Cell Biology Research, Internal/Organ Research, Research Tools

Description

MiR-193b^f/f mutant mice possess loxP sites flanking the entire microRNA 193b coding region making them useful in applications related to the regulation of brown adipose tissue differentiation.

Detailed description

MiR-193b^f/f mutant mice possess loxP sites flanking the entire microRNA 193b (miR-193b) coding region. MiR-193b is a microRNA involved in post-transcriptional regulation of genes expressed during brown adipose tissue (BAT) differentiation. Homozygous mice are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have miR-193b in the cre-expressing tissues.

For example, when bred to STOCK Tg(MMTV-cre)4Mam/J mice (Stock No. 003553) with widespread expression of Cre recombinase, these mice showed no overt phenotype, perhaps due to the compensatory expression of mir-133a1 and mir-133a2 alleles.

Development

A targeting vector was designed to insert a loxP site upstream, and a loxP-flanked and frt-flanked neomycin resistance (neo) cassette downstream of the microRNA 193b (miR-193b) coding region. The construct was electroporated into (C57BL/6N x 129S6/SvEvTac)F1-derived iTL embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. MiR-193b^f/f mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Mir193b^tm1.1Mam
Name: targeted mutation 1.1, Lothar Hennighausen
MGI accession ID: MGI:5517481
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mir193b
Marker name: microRNA 193b
Chromosome: 16
Strain of origin: (C57BL/6NTac x 129S6/SvEvTac)F1
Molecular note: A targeting vector was designed to insert a single loxP site upstream, and a loxP and FRT site flanked neomycin resistance gene cassette downstream of the locus. The neo cassette was removed by flpe-mediated recombination.