MiR-193bf/f mutant mice possess loxP sites flanking the entire microRNA 193b coding region making them useful in applications related to the regulation of brown adipose tissue differentiation.
Dr. Lothar Hennighausen, National Institutes of Health
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Mir193b | microRNA 193b |
MiR-193bf/f mutant mice possess loxP sites flanking the entire microRNA 193b (miR-193b) coding region. MiR-193b is a microRNA involved in post-transcriptional regulation of genes expressed during brown adipose tissue (BAT) differentiation. Homozygous mice are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have miR-193b in the cre-expressing tissues.
For example, when bred to STOCK Tg(MMTV-cre)4Mam/J mice (Stock No. 003553) with widespread expression of Cre recombinase, these mice showed no overt phenotype, perhaps due to the compensatory expression of mir-133a1 and mir-133a2 alleles.
A targeting vector was designed to insert a loxP site upstream, and a loxP-flanked and frt-flanked neomycin resistance (neo) cassette downstream of the microRNA 193b (miR-193b) coding region. The construct was electroporated into (C57BL/6N x 129S6/SvEvTac)F1-derived iTL embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. MiR-193bf/f mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Lothar Hennighausen |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | miR-193bf |
Gene Symbol and Name | Mir193b, microRNA 193b |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
Chromosome | 16 |
Molecular Note | A targeting vector was designed to insert a single loxP site upstream, and a loxP and FRT site flanked neomycin resistance gene cassette downstream of the locus. The neo cassette was removed by flpe-mediated recombination. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S6-Mir193btm1.1Mam/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #020100 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mir193b<tm1.1Lgp |
Frozen Mouse Embryo | B6;129S6-Mir193b<tm1.1Mam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Mir193b<tm1.1Mam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Mir193b<tm1.1Mam>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S6-Mir193b<tm1.1Mam>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.