These Fibulin-2 deficient mice exhibit better survival after experimental myocardial infarction when compared to wildtype controls and may be useful in studies of cardiac development, elastic fiber formation and homeostasis, and tumor cell growth and metastasis.
Mon-Li Chu, Thomas Jefferson University
Fibulin-2, an extracellular matrix glycoprotein found in elastic fibers and basement membranes, is involved in epithelial-mesenchymal transformation during development, angiogenesis, vascular wall maintenance, and is required for lung adenocarcinoma cells to grow and metastasize. Mice that are homozygous for this targeted mutation are viable and fertile. No gene product (mRNA or protein) is detected by Northern or Western blot analysis of mouse embryonic fibroblasts from homozygotes.
Homozygotes exhibit weakened TGF-beta signaling, better survival with less progressive ventricular dysfunction after experimental myocardial infarction when compared to wildtype controls. Homozygotes do not develop cardiac hypertrophy after angiotensin II treatment.
A targeting vector designed by Dr. Mon-Li Chu (Thomas Jefferson University) containing a loxP site flanked PGK-NEO cassette was inserted at codon 282 of exon 2. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Mon-Li Chu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Fbln2, fibulin 2|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A neo cassette was inserted after codon 282. The absences of transcript and protein were confirmed, respectively, by northern and western blot analysis.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S1-Fbln2tm1Chu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019895 in your Materials and Methods section.
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