Exon 2 of these Chek1 (checkpoint kinase 1) targeted mutant mice is flanked by loxP sites. When crossed with a tissue-specific Cre strain, the exon is excised, enabling selective disruption of protein production. This conditional allele may be helpful in the development of anticancer drug therapies.
Dr. Stephen Elledge, Harvard University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Chek1 | checkpoint kinase 1 |
Chek1 (checkpoint kinase 1) is a haploinsufficient tumor suppressor gene that is essential for embryonic stem cell survival. CHEK1 null mice reportedly exhibit embryonic lethality at E6.5 due to a peri-implantation defect. Activated CHEK1 prevents cell cycle progression by inhibiting the cell cycle phosphatases Cdc25a and Cdc25c.
Exon 2 of these targeted mutant mice is flanked by loxP sites. When crossed with a tissue-specific Cre strain, the exon is excised, enabling selective disruption of protein production.
When bred to mice carrying Tg(Wap-cre)11738Mam (see Stock No. 008735 for example), Cre recombinase expression in the mammary gland results in proliferating cells that undergo apoptosis leading to developmental defects. Conditional heterozygosity increases the number of S phase cells and causes spontaneous DNA damage.
This conditional allele may be helpful in the development of anticancer drug therapies.
A loxP-flanked neomycin cassette was ligated to a 2.2 kb exon 2-containing genomic fragment, of which the last 59 bp was replaced by an EcoRV and a loxP site. The targeting vector was introduced to 129-derived embryonic stem (ES) cells and the neomycin marker was excised through transient expression of Cre recombinase. This strain was backcrossed to C57BL/6 an estimated 5-6 times by the donating laboratory (see SNP note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. Two of the 27 markers throughout the genome originated from an unknown source (chromosome 11 at ~8.40 Mbpand chromosome 13 at ~21.4 Mbp). In addition, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating, suggesting the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1, Jeffrey M Rosen |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Chk1 Flox |
Gene Symbol and Name | Chek1, checkpoint kinase 1 |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 9 |
Molecular Note | LoxP sites were inserted around a 2.2kb genomic segment containing exon 2 via homologous recombination. |
Mutations Made By | Dr. Jeffrey Rosen, Baylor College of Medicine |
Heterozygous and homozygous floxed mice are viable and fertile.
When using the B6.129(Cg)-Chek1tm1Jmr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019520 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Chek1<tm1Jmr> |
Frozen Mouse Embryo | B6.129(Cg)-Chek1<tm1Jmr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Chek1<tm1Jmr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Chek1<tm1Jmr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Chek1<tm1Jmr>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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