These mice possess loxP sites flanking exon 3 of the G-protein signalling modulator 1 (AGS3-like, C. elegans), Gpsm1, gene and have applications in studies related to renal physiology, polycystic kidney disease, development, synaptic plasticity, cardiovascular regulation and energy homeostasis.
Stephen M. Lanier, Medical University of South Carolina
Heather A. Bainbridge, Medical University of South Carolina
G-protein signaling modulator 1 is a receptor independent activator of G protein signaling that is involved in cell division, autophagy, mitotic spindle orientation, protein trafficking, neuron plasticity and drug addiction behavior. These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
When bred to a strain with germline Cre recombinase expression (see Stock No. 003724 for example), this mutant mouse strain may be useful in studies of renal physiology, polycystic kidney disease, development, synaptic plasticity, cardiovascular regulation and energy homeostasis.
A targeting vector designed by Dr. Stephen Lanier (Medical University of South Carolina) was used to insert a single loxP site and an FRT flanked NEO cassette downstream of exon 3. A second single loxP site was inserted upstream of exon 3. This construct was electroporated into 129S6/SvEvTac derived W-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to transgenic mice (on the B6;SJL genetic background) expressing FLP1 recombinase under the control of the human ACTB promoter (Stock No. 003800). Mice that retained the loxP site flanked exon 3 were then bred to C57BL/6 mice for 13 generations. The mice no longer carry the NEO selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Stephen Lanier|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Gpsm1, G-protein signalling modulator 1 (AGS3-like, C. elegans)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A floxed and Frted PGK-neo cassette was inserted on one side and a single loxP sites was inserted on the other side of a 410 bp fragment that included exon 3. Cre- and Flp-mediated recombination resulted in removal of exon 3. Immunoblot analysis confirmed the absence of full length protein expression in brains and hearts from homozygous mice. Expression of a short form in the heart is not disrupted in homozygotes.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S6(SJL)-Gpsm1tm1.1Lajb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019503 in your Materials and Methods section.