B6.129P2(Cg)-Lepr^tm1.1Rck/J

Stock number: 019377
Research areas: Diabetes and Obesity Research, Reproductive Biology Research

Description

Homozygous Lepr (leptin receptor) knockout mice are morbidly obese, infertile and resemble Lepr^db-3J homozygous animals.

Detailed description

These Lepr (leptin receptor) mutant mice carry a knockout allele derived from the targeted deletion of exon 1. Heterozygotes are phenotypically normal, viable, and fertile. Homozygotes are morbidly obese, infertile and resemble Lepr^db-3J homozygous animals (also null for all Lepr isoforms). Plasma levels of leptin, glucose, insulin and corticosterone are elevated. Reported weights of males and females reach approximately 60g by 16 weeks of age.

Development

A single loxP site was inserted 5' of exon 1 and a loxP-flanked neomycin and thymidine kinase selection cassette was inserted 3' of exon 1 using 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. The floxed neomycin cassette was removed by transient Cre expression in the ES cells, leaving exon 1 flanked by loxP sites (see Stock No. 008327). Resultant mice were crossed with animals carrying an EIIa-cre to excise exon 1. This strain was backcrossed more than 10 times to C57BL/6J by the donating laboratory.

Allele

Symbol: Lepr^tm1.1Rck
Name: targeted mutation 1.1, Jeffrey M Friedman
MGI accession ID: MGI:2176775
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Lepr
Marker name: leptin receptor
Chromosome: 4
Strain of origin: 129P2/OlaHsd
Molecular note: This allele is a derivative of Lepr^tm1.1Rck in which exon 1 and flanking sequences were deleted in the germline by crossing mice carrying this allele to mice carrying Tg(EIIa-Cre)1Lmgd.