FVB/N-Tg(tetO-MYC)36aBop/J

Stock number: 019376
Product name: tet-o-MYC line 36
Strain synonyms: TetO-c-Myc, TRE-MYC
Research areas: Cancer Research, Research Tools

Description

These TRE-MYC (TetO-c-Myc or Teto-MYC) hemizygous transgenic mice carry the human myelocytomatosis oncogene, MYC, regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of MYC protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.These mice may be useful for studying tumor development and regression.

 

Detailed description

Expression of MYC in these transgenic mice is regulated by a tetracycline operator (tetO). MYC is a transcription factor involved in cellular proliferation and differentiation, and is often upregulated in tumors, leukemias and lymphomas. Hemizygous mice are viable and fertile, homozygotes do not survive. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of MYC protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.

When bred to B6.Cg-Tg(Cebpb-tTA)5Bjd/J mice (as Stock No. 003563) expressing tTA in liver, withdrawal of dox from double transgenic mice develop invasive metastatic liver tumors, and all mice die within 2 weeks onset. When doxycycline treatment is re-administered, rapid and sustained tumor regression is evident.

When bred to B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J (see Stock No. 007176) expressing rtTA in renal tubular epithelial cells, induction of doxycycline causes polycystic kidneys, kidney failure and renal adenomas.

Of note: in B6.FVB-Tg(tetO-MYC)36aBop/DwfJ (Stock No. 034532) the tet-o-MYC transgene inserted on the Y chromosome and is transmitted only through males.

Development

A transgenic vector was generated encoding the human myelocytomatosis viral oncogene cDNA (MYC) under control of a tetO promoter. The construct was microinjected into FVB/NJ fertilized oocytes, and mice from founder line 36a were bred to FVB/NJ mice for at least 10 generations to establish a colony. Upon arrival at The Jackson Laboratory Repository, mice were bred to FVB/NJ mice (as Stock No. 001800) for at least one generation.

Allele

Symbol: Tg(tetO-MYC)36aBop
Name: transgene insertion 36a, J Michael Bishop
MGI accession ID: MGI:3039697
Generation method: Transgenic
Attributes: Inducible; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(tetO-MYC)36aBop
Marker name: transgene insertion 36a, J Michael Bishop
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: MYC,MYC proto-oncogene, bHLH transcription factor,human
Molecular note: This transgene contains a human v-myc avian myelocytomatosis viral oncogene homolog cDNA and regulatory elements from the bacterial tetracycline-resistance operon. There are two founder #36 lines. Transgene insertions are on an autosomal chromosome in this line.
General note: Phenotypic Similarity to Human Syndrome: Renal Cell Carcinoma J:222943.