These TRE-MYC (TetO-c-Myc or Teto-MYC) hemizygous transgenic mice carry the human myelocytomatosis oncogene, MYC, regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of MYC protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.These mice may be useful for studying tumor development and regression.
J Michael Bishop, UCSF School of MedicineRead More +
Expression of MYC in these transgenic mice is regulated by a tetracycline operator (tetO). MYC is a transcription factor involved in cellular proliferation and differentiation, and is often upregulated in tumors, leukemias and lymphomas. Hemizygous mice are viable and fertile, homozygotes do not survive. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of MYC protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.
When bred to B6.Cg-Tg(Cebpb-tTA)5Bjd/J mice (as Stock No. 003563) expressing tTA in liver, withdrawal of dox from double transgenic mice develop invasive metastatic liver tumors, and all mice die within 2 weeks onset. When doxycycline treatment is re-administered, rapid and sustained tumor regression is evident.
When bred to B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J (see Stock No. 007176) expressing rtTA in renal tubular epithelial cells, induction of doxycycline causes polycystic kidneys, kidney failure and renal adenomas.
A transgenic vector was generated encoding the human myelocytomatosis viral oncogene cDNA (MYC) under control of a tetO promoter. The construct was microinjected into FVB/NJ fertilized oocytes, and mice from founder line 36a were bred to FVB/NJ mice for at least 10 generations to establish a colony. Upon arrival at The Jackson Laboratory Repository, mice were bred to FVB/NJ mice (as Stock No. 001800) for at least one generation.
|Expressed Gene||MYC, v-myc avian myelocytomatosis viral oncogene homolog, human|
|Site of Expression|
|Allele Name||transgene insertion 36a, J Michael Bishop|
|Allele Type||Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)|
|Allele Synonym(s)||TRE-MYC; TetO-c-Myc; Teto-MYC; Tg(tetO-MYC)36Bop|
|Gene Symbol and Name||Tg(tetO-MYC)36aBop, transgene insertion 36a, J Michael Bishop|
|Promoter||tetO, tet operator,|
|Expressed Gene||MYC, v-myc avian myelocytomatosis viral oncogene homolog, human|
|Strain of Origin||FVB/N|
|General Note||Phenotypic Similarity to Human Syndrome: Renal Cell Carcinoma J:222943.|
|Molecular Note||This transgene contains a human v-myc avian myelocytomatosis viral oncogene homolog cDNA and regulatory elements from the bacterial tetracycline-resistance operon. There are two founder #36 lines. Transgene insertions are on an autosomal chromosome inthis line.|
|Mutations Made By|| |
J Michael Bishop, UCSF School of Medicine
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to FVB/NJ inbred mice (as Stock No. 001800). Homozygous mice do not survive.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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