These PTIP-floxed mice possess loxP sites flanking exon 1 of the PAX interacting (with transcription-activation domain) protein 1 (Paxip1) gene and have applications in studies related to early embryonic development and transcriptional regulation.
Gregory R Dressler, University of Michigan
Paxip1, PAX interacting (with transcription-activation domain) protein 1, (PTIP) associates with histone H3K4 methyltransferases and is involved in DNA damage response, transcriptional regulation and progression through mitosis. These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in the renal inner medulla, this mutant mouse strain may be useful in studies of renal physiology, urine concentration and osmotic tolerance.
A targeting vector containing a FRT site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site was inserted downstream of exon 1. This construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 X (C57BL/6 X DBA/2)F1 blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6 mice. The mice were then crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP1 recombinase to remove the selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Gregory R Dressler|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||ptipfl; PTIPflox; ptiploxP|
|Gene Symbol and Name||Paxip1, PAX interacting (with transcription-activation domain) protein 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neo cassette flanked by FRT sites was inserted upstream of exon 1 and an additional loxP site was inserted downstream of exon 1. The neo cassette was subsequently removed by flp-mediated recombination.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Paxip1tm2Gdr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #019143 in your Materials and Methods section.