Overview

This strain provides an asplenic NOD that develops diabetes with the same incidence as standard NOD mice.

Donating Investigator

Dr. David Serreze, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
001976 NOD/ShiLtJ

Tlx1^tm1Sjk

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Tlx1	T cell leukemia, homeobox 1

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Research Applications

Diabetes and Obesity Research
Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Developmental Biology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the Tlx1^tm1Sjk targeted mutation on this NOD congenic background do not differ in diabetes incidence from standard NOD controls and thymic T and B cell profiles are normal even though these homozygotes are asplenic. The outward appearance looks like that of the NOD host background, with no obvious limb abnormalities (D.V. Serreze, personal communication). Tlx1^tm1Sjk homozygotes have been finely characterized on other genetic backgrounds and found to lack a spleen; however, all other internal organs were found to be normal. Characteristics reported included: polydactyly or oligodactyly of the hindlimbs, tibial hemimelia, and sometimes reduction of the femur and pubic element of the pelvic girdle. Pharyngeal and mastication muscles derived from the branchial arches were reported to be present and anatomically normal homozygotes had normal cranial ganglia morphology and position and normal cranial motor nuclei function. Also reported were normal numbers of RBCs but increased numbers of WBCs, including neutrophils and lymphocytes. The B-cell and T-cell profile was described as normal in the thymus, lymph nodes and peripheral blood. Many erythrocytes had nuclear fragments (Howell-Jolly bodies).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

This congenic strain was generated by Dr. David Serreze at The Jackson Laboratory. The Tlx1^tm1Sjk allele was backcrossed from the strain B6.129S2-Tlx1^tm1Sjk/J (stock #002771) onto the NOD/ShiLtDvs background for 10 generations before sibling inbreeding to homozygosity. Sperm was cryopreserved from homozygous males at generation N10F5 and NOD/ShiLtJ females are used for recovery by in vitro fertilization.

Control Suggestions

Approximate Controls

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Roberts CW; Shutter JR; Korsmeyer SJ. 1994. Hox11 controls the genesis of the spleen. Nature 368(6473):747-9PubMed: 7908720 MGI: J:17695

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Genetics

Tlx1^tm1Sjk

Allele Symbol: Tlx1^tm1Sjk

Allele Name	targeted mutation 1, Stanley J Korsmeyer
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Hox11^-; Tlx-; Tlx1^-
Gene Symbol and Name	Tlx1, T cell leukemia, homeobox 1
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	19
Molecular Note	Exon 1 was disrupted by the insertion of a neomycin selection cassette into codon 53. Transcript was undetected by in situ hybridization of homozygous mutant mice.
Mutations Made By	Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM) Analysis Strains
- Type 1 Diabetes (IDDM)

Genotype: Tlx1^tm1Sjk related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
Internal/Organ Research
- Spleen Defects
  - asplenic
Developmental Biology Research
- Internal/Organ Defects
  - asplenic

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tlx1^tm1Sjk/Tlx1^tm1Sjk
involves: 129S2/SvPas

hematopoietic system phenotype

abnormal spleen development
- at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development
- at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium

abnormal spleen mesenchyme morphology
- at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen

absent spleen
- all adult homozygotes appear externally normal but display complete absence of spleen
- Normal - in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position

increased leukocyte cell number
- homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice
- Normal - however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood

increased lymphocyte cell number
- in peripheral blood smears

increased neutrophil cell number
- in peripheral blood smears

increased number of Howell-Jolly bodies
- Normal - however, complete blood counts indicate that the number of red blood cells is normal
- many erythrocytes contain nuclear fragments of condensed DNA known as Howell-Jolly bodies, commonly associated with asplenia

immune system phenotype

abnormal spleen development
- at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development
- at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium

abnormal spleen mesenchyme morphology
- at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen

absent spleen
- all adult homozygotes appear externally normal but display complete absence of spleen
- Normal - in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position

increased leukocyte cell number
- homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice
- Normal - however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood

increased lymphocyte cell number
- in peripheral blood smears

increased neutrophil cell number
- in peripheral blood smears

mammalian phenotype

embryo phenotype
- Normal - despite observed gene expression in the branchial region of wild-type mice, homozygotes display no detectable abnormalities in branchial arches

nervous system phenotype
- Normal - despite observed gene expression in the developing hindbrain and cranial ganglia of wild-type mice, homozygotes display no detectable abnormalities in the hindbrain, cranial ganglia or cranial motor nuclei

References

Roberts CW; Shutter JR; Korsmeyer SJ. 1994. Hox11 controls the genesis of the spleen. Nature 368(6473):747-9PubMed: 7908720 MGI: J:17695

Additional - Tlx1^tm1Sjk related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tlx1alternate1
- Genotyping resources and troubleshooting
Appearance
- albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.129S2(B6)-Tlx1^tm1Sjk/DvsJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #019142 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Tlx1<tm1Sjk>

Payment Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tlx1tm1Sjk

Allele Symbol: Tlx1tm1Sjk

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Tlx1tm1Sjk related

Mammalian Phenotype Terms by Genotype

Genotype: Tlx1tm1Sjk/Tlx1tm1Sjkinvolves: 129S2/SvPas

hematopoietic system phenotype

immune system phenotype

mammalian phenotype

References

Additional - Tlx1tm1Sjk related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tlx1^tm1Sjk

Allele Symbol: Tlx1^tm1Sjk

Genotype: Tlx1^tm1Sjk related

Genotype: Tlx1^tm1Sjk/Tlx1^tm1Sjk
involves: 129S2/SvPas

Additional - Tlx1^tm1Sjk related